Ethidium bromide
Synonyms: Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide
SECTION 1: Identification of the substance/mixture and of the company/undertaking
Chemical Name
Ethidium bromide
Product Form
Substance
Catalogue No.
AL0675B
CAS Number
1239-45-8
EC Number
214-200-4
EC Index Number
611-007-00-2
Grade
AR grade
HSN Code
29339900
Molecular Formula
C21H20BrN3
SECTION 1: Identification of the substance/mixture and of the company/undertaking
1.1 Product identifier
Chemical Name
Ethidium bromide
Catalogue No.
AL0675B
Product form
Substance
CAS Number
1239-45-8
EC Number
214-200-4
Grade
AR grade
EC Index Number
611-007-00-2
Chemical Formula
C21H20BrN3
HSN Code
29339900
Synonyms
Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide
Chemical Structure
-
1.2 Relevant identified uses of the substance or mixture and uses advised against
Use of the substance/mixture
Laboratory reagent for DNA/RNA visualization
Uses advised against
Not specified
1.3 Details of the supplier of the safety data sheet
Company identification
Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 Emergency Contact Details
Phone / e-Mail
Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
SECTION 2: Hazards identification
2.3 Other hazards
Other hazards
Mutagenic and suspected carcinogen; handle with care.
SECTION 3: Composition/information on ingredients
3.1 Substance
Name
Ethidium bromide
CAS No.
1239-45-8
SECTION 4: First aid measures
4.1 Description of first aid measures
First-aid measures after inhalation
Move to fresh air. If symptoms persist, seek medical attention.
First-aid measures after skin contact
Wash with soap and water. Seek medical attention if irritation develops.
First-aid measures after eye contact
Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
First-aid measures after ingestion
Do not induce vomiting. Rinse mouth and seek medical attention immediately.
4.2 Most important symptoms and effects, both acute and delayed
Symptoms relating to use
Symptoms of exposure may include irritation of the respiratory tract, skin, and eyes; nausea; and potential mutagenic effects.
4.3 Indication of any immediate medical attention and special treatment needed
General information
Treat symptomatically. Show this safety data sheet to the treating physician.
SECTION 5: Fire-fighting measures
5.1 Extinguishing media
Suitable extinguishing media
Water spray, carbon dioxide, dry chemical powder, foam.
Unsuitable extinguishing media
Not specified
5.2 Special hazards arising from the substance or mixture
Special procedures
Toxic fumes may be released in a fire. Use water spray to cool containers.
5.3 Advice for firefighters
Protection during firefighting
Do not enter fire area without proper protective equipment, including respiratory protection.
SECTION 6: Accidental release measures
6.1.1 For non-emergency personnel
Emergency procedures
Evacuate area and ventilate. Wear appropriate protective equipment. Contain spill and collect for disposal.
6.3 Methods and material for containment and cleaning up
Methods for cleaning up
Use appropriate personal protective equipment. Absorb spill with inert material and dispose of as hazardous waste.
6.4 Reference to other sections
Reference
Not specified
SECTION 7: Handling and storage
7.1 Precautions for safe handling
Precautions for safe handling
Avoid contact with skin and eyes. Use appropriate personal protective equipment (PPE) including gloves and goggles.
Hygiene measures
Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 Conditions for safe storage, including any incompatibilities
Storage conditions
Store in a tightly closed container in a cool, dry, well-ventilated area away from incompatible materials.
SECTION 8: Exposure controls/personal protection
8.2 Exposure controls
Hand protection
Protective gloves (e.g. nitrile rubber)
Eye protection
Chemical safety goggles or safety glasses
Skin and body protection
Wear suitable protective clothing
Respiratory protection
Use a NIOSH-approved respirator if exposure limits are exceeded or if irritation occurs.
SECTION 9: Physical and chemical properties
9.1 Information on basic physical and chemical properties
Physical state
Solid
Colour
Dark red-purple
Odour
Odourless
Odour threshold
No data available
pH value
No data available
Relative evaporation rate (butylacetate=1)
No data available
Melting point
110-112 °C
Freezing point
No data available
Boiling point
250 °C
Flash point
No data available
Auto-ignition temperature
No data available
Decomposition temperature
No data available
Flammability (solid, gas)
No
Vapour pressure
No data available
Relative vapour density at 20°C
No data available
Density
1.5 g/cm³
Solubility
Soluble in water
Log Pow
No data available
Viscosity, kinematic
No data available
Viscosity, dynamic
No data available
Explosive properties
Not explosive
Oxidising properties
Not oxidizing
Explosive limits
No data available
SECTION 10: Stability and reactivity
10.1 Reactivity
Reactivity
Stable under recommended storage conditions. Avoid strong oxidizing agents.
10.2 Chemical stability
Chemical stability
Stable under recommended storage conditions.
10.3 Possibility of hazardous reactions
Possibility of hazardous reactions
No dangerous reactions known under normal conditions of use.
10.4 Conditions to avoid
Conditions to avoid
Avoid contact with incompatible materials and excessive heat.
10.5 Incompatible materials
Incompatible materials
Strong oxidizing agents, strong acids, and bases.
10.6 Hazardous decomposition products
Hazardous decomposition products
Not specified
SECTION 11: Toxicological information
11.1 Information on toxicological effects
Acute toxicity
LD50 (oral, rat) = 10 mg/kg
Skin corrosion/irritation
Causes skin irritation.
Serious eye damage/irritation
Causes serious eye irritation.
Respiratory or skin sensitisation
May cause sensitization by skin contact.
Germ cell mutagenicity
Not classified
Carcinogenicity
Not classified
Specific target organ toxicity (single exposure)
May cause respiratory irritation.
Specific target organ toxicity (repeated exposure)
Not classified
SECTION 12: Ecological information
12.1 Toxicity
Toxicity information
Not specified
12.2 Persistence - degradability
Persistence - degradability
Not specified
12.3 Bioaccumulative potential
Bioaccumulative potential
Not specified
12.4 Mobility in soil
Mobility in soil
Not specified
12.6 Other adverse effects
Environmental precautions
Not specified
SECTION 13: Disposal considerations
13.1 Waste treatment methods
Additional Info
Dispose of contents/container in accordance with local, regional, national and international regulations.
SECTION 14: Transport information
14.1 UN number
UN-No. (ADR)
2811
UN-No. (IMDG)
2811
UN-No. (IATA)
2811
UN-No. (ADN)
2811
UN-No. (RID)
2811
14.2 UN proper shipping name
Proper Shipping Name (ADR)
Toxic solid, organic, n.o.s. (Ethidium bromide)
Proper Shipping Name (IMDG)
Toxic solid, organic, n.o.s. (Ethidium bromide)
Proper Shipping Name (IATA)
Toxic solid, organic, n.o.s. (Ethidium bromide)
Proper Shipping Name (ADN)
Toxic solid, organic, n.o.s. (Ethidium bromide)
Proper Shipping Name (RID)
Toxic solid, organic, n.o.s. (Ethidium bromide)
14.3 Transport hazard class(es)
Transport hazard class(es)
6.1
14.4 Packing group
Packing group (ADR)
I
Packing group (IMDG)
I
Packing group (IATA)
I
Packing group (ADN)
I
Packing group (RID)
I
14.5 Environmental hazards
Dangerous for the environment
Not specified
Marine pollutant
Not specified
Other information
No supplementary information available
SECTION 15: Regulatory information
15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture
15.1.1 EU-Regulations
Not specified
SECTION 16: Other Information
Disclaimer
The information provided in this Safety Data Sheet is correct to the best of our knowledge, information and belief at the date of its publication. The information is designed only as a guidance for safe handling, use, processing, storage, transportation, disposal and release and is not to be considered a guarantee or quality specification. The information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process, unless specified in the text.