2,3,5-Triphenyltetrazolium chloride
- CAS Number
- 298-96-4
- HSN Code
- 29339900
- Molecular Formula
- C19H15ClN4
- Catalogue Number
- AL1500A
- Purity
- ≥ 99%
- Molecular Weight
- 334.81 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Tetrazolium salt (AR, TTC, 2,3,5-triphenyltetrazolium chloride) is a widely used redox indicator dye that turns red upon reduction by living cells. This compound is notable for its application in various biological assays, particularly in assessing cell viability and metabolic activity. It is classified as an analytical reagent (AR) with a purity of 99%, making it suitable for laboratory use. Tetrazolium salts are essential in microbiology and plant biology, providing a visual indication of cellular activity and health.
Also known as TTC, Tetrazolium chloride.
- Catalogue No.
- AL1500A
- CAS Number
- 298-96-4
- Molecular Formula
- C19H15ClN4
- Molecular Weight
- 334.81 g/mol
- HSN Code
- 29339900
- Grade
- AR (Analytical Reagent)
- Product Form
- Substance
Tetrazolium salt appears as a white to pale yellow crystalline powder that is light-sensitive. It has a melting point of approximately 150-155 °C and is soluble in water, with a solubility of about 10 g/L at room temperature. The compound has a density of 1.25 g/cm³ and is known to be a flammable solid. Its vapour pressure is low, indicating stability under normal conditions, but it should be stored away from light to prevent degradation.
- Appearance
- White to pale yellow crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g glass bottles
- Storage
- Store at 2-8°C, protected from light.
1.1 — Product identifier
- Chemical Name
- 2,3,5-Triphenyltetrazolium chloride
- Catalogue No.
- AL1500A
- Product form
- Substance
- CAS Number
- 298-96-4
- EC Number
- 206-059-0
- Grade
- AR
- EC Index Number
- N/A
- Chemical Formula
- C19H15ClN4
- HSN Code
- 29339900
- Synonyms
- TTC, Tetrazolium chloride
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Redox indicator dye, cell viability indicator
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Flammable solid; light-sensitive
3.1 — Substance
- Name
- 2,3,5-Triphenyltetrazolium chloride
- CAS No.
- 298-96-4
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Seek medical attention if irritation persists.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; potential for allergic reactions.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Flammable; may produce toxic fumes in a fire.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Evacuate area and ventilate. Use appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Collect and dispose of in accordance with local regulations. Avoid release into the environment.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area. Keep away from heat and open flames.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from light and incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- White to pale yellow
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 150-155 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Flammable solid
- Vapour pressure
- Low
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.25 g/cm³
- Solubility
- 10 g/L in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidising
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; avoid exposure to light.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids, and bases.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; may cause irritation.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause irritation.
- Respiratory or skin sensitisation
- Not expected to be a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- UN 1325
- UN-No. (IMDG)
- UN 1325
- UN-No. (IATA)
- UN 1325
- UN-No. (ADN)
- UN 1325
- UN-No. (RID)
- UN 1325
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Tetrazolium salt
- Proper Shipping Name (IMDG)
- Tetrazolium salt
- Proper Shipping Name (IATA)
- Tetrazolium salt
- Proper Shipping Name (ADN)
- Tetrazolium salt
- Proper Shipping Name (RID)
- Tetrazolium salt
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 4.1
14.4 — Packing group
- Packing group (ADR)
- II
- Packing group (IMDG)
- II
- Packing group (IATA)
- II
- Packing group (ADN)
- II
- Packing group (RID)
- II
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Tetrazolium salt is primarily used as a cell-viability indicator in seed-viability testing and microbiology. It serves as a crucial component in culture media for bacterial growth, allowing researchers to assess the metabolic activity of microorganisms. Additionally, it is employed in various biochemical assays to evaluate the health of cells in tissue culture and to monitor the effects of drugs on cell viability. Its versatility extends to applications in plant biology, where it helps in determining seed germination potential and overall plant health.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1500A
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 334.81 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Buff colored powder | Buff colored powder |
| Assay | (Min.) 99% | 99.51% |
| Heavy metals (as Pb) | <0.001% | <0.001% |
| Iron (Fe) | <0.001% | 0.0008% |
| Sulphated ash | <0.2% | 0.1% |
| Sensitivity to glucose | 1:150000 | 1:150000 |
The above product complies with AR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| 99% AR | ≥ 99% | 150-155 °C | ≤ 0.5% | ≤ 0.1% | None detected | 10 g/L in water |
Related Products
View AllWhy buy from Alpha Chemika
What Our Scientific & Industrial Customers Say
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
FAQs
Common Questions About Laboratory Chemicals & Solutions
Find answers to common questions about laboratory chemicals, product selection, documentation, ordering, and technical support. Whether you are sourcing chemicals for research, testing, education, manufacturing, or industrial applications, these FAQs can help you make informed purchasing decisions.
Alpha Chemika offers a wide range of laboratory chemicals, reagents, solvents, and specialty products for research, testing, educational, pharmaceutical, and industrial applications.
Product selection depends on factors such as application, required purity, grade, specifications, and intended use. Our team can help you identify suitable products based on your technical requirements.
Yes. Relevant product information and technical documentation can be provided to help you evaluate products for quality, handling, compliance, and application requirements.
Yes. You can share your product list, required quantities, specifications, and delivery requirements with our team for a tailored quotation.
Yes. We support requirements from research laboratories, educational institutions, pharmaceutical companies, manufacturers, distributors, and other scientific and industrial organizations.
Connect with Us for Your Requirements
Have a specific product requirement or need help finding the right chemical for your application? Share your requirements with our team, and we will help you identify suitable products, specifications, quantities, and procurement options.
