New Methylene Blue
- CAS Number
- 6586-05-6
- HSN Code
- 32041351
- Molecular Formula
- C18H22ClN3S·1/2ZnCl2
- Catalogue Number
- AL1061B
- Purity
- Min. 90%
- Molecular Weight
- 416.05 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
New methylene blue (certified) is a thiazine dye utilized primarily as a vital stain, particularly for reticulocyte counting in veterinary hematology. It is recognized for its high purity and specific dye content, making it suitable for laboratory applications.
Also known as C.I. No. 52030, Methylene Blue, Thionine.
- Catalogue No.
- AL1061B
- CAS Number
- 6586-05-6
- Molecular Formula
- C18H22ClN3S·1/2ZnCl2
- Molecular Weight
- 416.05 g/mol
- HSN Code
- 32041351
- Grade
- Certified
- Product Form
- Substance
Dark blue-green crystalline powder with a characteristic color indicative of its dye properties. It is soluble in water and exhibits stability under normal laboratory conditions.
- Appearance
- Dark blue-green crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g glass bottles
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- New Methylene Blue
- Catalogue No.
- AL1061B
- Product form
- Substance
- CAS Number
- 6586-05-6
- EC Number
- 229-851-5
- Grade
- Certified
- EC Index Number
- Not applicable
- Chemical Formula
- C18H22ClN3S·1/2ZnCl2
- HSN Code
- 32041351
- Synonyms
- C.I. No. 52030, Methylene Blue, Thionine
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Used as a vital stain in hematology and biological research.
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause respiratory irritation; avoid inhalation of dust.
3.1 — Substance
- Name
- New Methylene Blue
- CAS No.
- 6586-05-6
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of the respiratory tract, skin, and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes under fire conditions.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Sweep up and place in a suitable container for disposal.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid inhalation and contact with skin. Use gloves and a dust mask when handling.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a dust mask or respirator if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark blue-green
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.2 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Not classified as acutely toxic.
- Skin corrosion/irritation
- May cause skin irritation.
- Serious eye damage/irritation
- May cause eye irritation.
- Respiratory or skin sensitisation
- Not classified as a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
This dye is extensively used in hematology for reticulocyte counting and general blood-cell staining, aiding in the diagnosis and study of various blood disorders.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1061B
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 416.05 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay (Dye Content) | Min. 90% | 92% |
| Moisture Content | Max. 5% | 3.2% |
| Residue on Ignition | Max. 1% | 0.5% |
| Related Substances | None detected | None detected |
| Solubility | Soluble in water | Fully soluble in water at room temperature. |
The above product is expected to comply with Certified grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Certified | Min. 90% | Not applicable | Max. 5% | Max. 1% | None detected | Soluble in water |
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