Rhodamine B
- CAS Number
- 81-88-9
- HSN Code
- 32041342
- Molecular Formula
- C28H31ClN2O3
- Catalogue Number
- AL01289
- Purity
- Min. 85%
- Molecular Weight
- 479.01 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Rhodamine B (AR, M.S.) is a synthetic dye belonging to the xanthene class, renowned for its vibrant pink-red fluorescence. It is widely utilized in various applications, particularly in the fields of biology and chemistry for tracing and labeling due to its high visibility and stability. This compound is characterized by its purity of at least 85% and is commonly referenced by its C.I. number 45170. Rhodamine B is also notable for its molecular weight of 479.01 g/mol and is frequently employed in research and industrial applications requiring fluorescent markers.
Also known as C.I. 45170, Basic Rhodamine B.
- Catalogue No.
- AL01289
- CAS Number
- 81-88-9
- Molecular Formula
- C28H31ClN2O3
- Molecular Weight
- 479.01 g/mol
- HSN Code
- 32041342
- Grade
- AR, M.S.
- Product Form
- Substance
Rhodamine B appears as a dark red-purple crystalline powder with a distinctive green sheen. It has a melting point of approximately 200 °C and is soluble in water, making it suitable for various aqueous applications. The compound exhibits a strong fluorescence under UV light, which is a key property for its use in tracing and labeling. Its density is around 1.2 g/cm³, and it has a boiling point of approximately 600 °C, indicating its stability at high temperatures. The dye is also known to have a pH value in solution of around 4.5 to 6.5, which is optimal for its fluorescent properties.
- Appearance
- Dark red-purple crystalline powder with a green sheen
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g glass bottles
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Rhodamine B
- Catalogue No.
- AL01289
- Product form
- Substance
- CAS Number
- 81-88-9
- EC Number
- 201-165-7
- Grade
- AR, M.S.
- EC Index Number
- -
- Chemical Formula
- C28H31ClN2O3
- HSN Code
- 32041342
- Synonyms
- C.I. 45170, Basic Rhodamine B
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Fluorescent dye for tracing and labeling applications
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Suspected carcinogen; handle with care.
3.1 — Substance
- Name
- Rhodamine B
- CAS No.
- 81-88-9
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Seek medical attention if irritation persists.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; potential carcinogenic effects.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes when heated. Use appropriate fire-fighting measures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use absorbent materials to clean up spills and dispose of in accordance with local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use personal protective equipment as required.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from light and incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use in a well-ventilated area. If exposure limits are exceeded, use appropriate respiratory protection.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red-purple
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- 4.5 - 6.5 (in solution)
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 200 °C
- Freezing point
- Not applicable
- Boiling point
- 600 °C
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable under normal conditions
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.2 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- LD50 (oral, rat) > 2000 mg/kg; low toxicity.
- Skin corrosion/irritation
- May cause skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- May cause allergic skin reactions in sensitive individuals.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Rhodamine B is extensively used as a fluorescent tracer dye in hydrology studies, particularly for water tracing applications where it helps in tracking the movement of water through various environments. It is also employed in microbiology for staining cells and tissues, enhancing the visibility of biological samples under fluorescence microscopy. Additionally, Rhodamine B serves as a reference compound in laser dye applications, contributing to the development of laser technologies. Its vibrant color and fluorescent properties make it valuable in the textile industry for dyeing processes, as well as in the production of inks and paints.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01289
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 479.01 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay | Min. 85% | 87% |
| Melting Point | 200 °C | 201 °C |
| Moisture Content | Max. 5% | 3.2% |
| Residue on Ignition | Max. 1% | 0.5% |
| Related Substances | None detected | None detected |
| Solubility | Soluble in water | Fully soluble in water at room temperature. |
The above product is expected to comply with AR, M.S. grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| AR, M.S. | Min. 85% | 200 °C | Max. 5% | Max. 1% | None detected | Soluble in water |
Related Products
View AllWhy buy from Alpha Chemika
What Our Scientific & Industrial Customers Say
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
FAQs
Common Questions About Laboratory Chemicals & Solutions
Find answers to common questions about laboratory chemicals, product selection, documentation, ordering, and technical support. Whether you are sourcing chemicals for research, testing, education, manufacturing, or industrial applications, these FAQs can help you make informed purchasing decisions.
Alpha Chemika offers a wide range of laboratory chemicals, reagents, solvents, and specialty products for research, testing, educational, pharmaceutical, and industrial applications.
Product selection depends on factors such as application, required purity, grade, specifications, and intended use. Our team can help you identify suitable products based on your technical requirements.
Yes. Relevant product information and technical documentation can be provided to help you evaluate products for quality, handling, compliance, and application requirements.
Yes. You can share your product list, required quantities, specifications, and delivery requirements with our team for a tailored quotation.
Yes. We support requirements from research laboratories, educational institutions, pharmaceutical companies, manufacturers, distributors, and other scientific and industrial organizations.
Connect with Us for Your Requirements
Have a specific product requirement or need help finding the right chemical for your application? Share your requirements with our team, and we will help you identify suitable products, specifications, quantities, and procurement options.
