Robert's Reagent Solution
- CAS Number
- -
- HSN Code
- 38229090
- Molecular Formula
- HNO3/(NH4)2SO4
- Catalogue Number
- AL1290F
- Purity
- N/A
- Molecular Weight
- N/A
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Robert's reagent solution is a classic clinical chemistry reagent used for the qualitative detection of protein (albumin) in urine. This reagent is a mixture of nitric acid and ammonium sulfate, which reacts with proteins to form a colored complex, indicating the presence of protein in the sample. It is widely utilized in clinical laboratories for routine urinalysis, particularly in the diagnosis of kidney disorders. The solution is ready-to-use, ensuring convenience and reliability in testing procedures.
Also known as Nitric acid/ammonium sulfate mixture.
- Catalogue No.
- AL1290F
- CAS Number
- -
- Molecular Formula
- HNO3/(NH4)2SO4
- Molecular Weight
- N/A
- HSN Code
- 38229090
- Grade
- Ready-to-use reagent solution
- Product Form
- Mixture
Robert's reagent solution is a clear, colorless liquid, indicative of its purity and lack of particulate matter. The density of the solution is approximately 1.2 g/cm³, which is typical for concentrated nitric acid solutions. It has a boiling point of around 83°C, corresponding to the boiling point of nitric acid, and a freezing point of approximately -42°C. The solution is highly soluble in water, allowing for easy preparation and application in laboratory settings.
- Appearance
- Clear, colorless liquid
- Shelf Life
- 12 months
- Packaging
- Available in 100 mL and 500 mL bottles
- Storage
- Store in a cool, dry place away from direct sunlight and incompatible materials.
1.1 — Product identifier
- Chemical Name
- Robert's Reagent Solution
- Catalogue No.
- AL1290F
- Product form
- Mixture
- CAS Number
- Not applicable
- EC Number
- Not applicable
- Grade
- Ready-to-use reagent solution
- EC Index Number
- Not applicable
- Chemical Formula
- HNO3/(NH4)2SO4
- HSN Code
- 38229090
- Synonyms
- Nitric acid/ammonium sulfate mixture
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Qualitative detection of protein in urine
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Corrosive to metals
3.1 — Substance
- Name
- Robert's Reagent Solution
- CAS No.
- Not applicable
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash skin thoroughly with soap and water. Seek medical attention if irritation develops.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Seek medical attention.
- First-aid measures after ingestion
- Do not induce vomiting. Rinse mouth and seek medical attention immediately.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Corrosive effects may include irritation, redness, and burns on contact with skin or eyes; respiratory irritation if inhaled.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Non-flammable, but may cause fire and explosion in contact with organic materials.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Wear appropriate protective equipment. Neutralize with a suitable base before disposal.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Use personal protective equipment as required. Avoid contact with skin and eyes. Ensure adequate ventilation.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible substances such as bases and organic materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if ventilation is inadequate.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Colorless
- Odour
- Pungent, characteristic of nitric acid
- Odour threshold
- Not established
- pH value
- <1 (highly acidic)
- Relative evaporation rate (butylacetate=1)
- Not established
- Melting point
- -42°C
- Freezing point
- Not established
- Boiling point
- 83°C
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not established
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not established
- Relative vapour density at 20°C
- Not established
- Density
- 1.2 g/cm³
- Solubility
- Fully miscible in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not established
- Viscosity, dynamic
- Not established
- Explosive properties
- Not explosive
- Oxidising properties
- Strong oxidizer
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Reacts violently with bases and organic materials, releasing toxic gases.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong bases, organic materials, reducing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Harmful if inhaled or swallowed; may cause burns.
- Skin corrosion/irritation
- Causes severe skin burns and eye damage.
- Serious eye damage/irritation
- Causes serious eye damage.
- Respiratory or skin sensitisation
- Not a skin sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- May cause respiratory irritation.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- UN 2031
- UN-No. (IMDG)
- UN 2031
- UN-No. (IATA)
- UN 2031
- UN-No. (ADN)
- UN 2031
- UN-No. (RID)
- UN 2031
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Nitric acid solution
- Proper Shipping Name (IMDG)
- Nitric acid solution
- Proper Shipping Name (IATA)
- Nitric acid solution
- Proper Shipping Name (ADN)
- Nitric acid solution
- Proper Shipping Name (RID)
- Nitric acid solution
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 8
14.4 — Packing group
- Packing group (ADR)
- II
- Packing group (IMDG)
- II
- Packing group (IATA)
- II
- Packing group (ADN)
- II
- Packing group (RID)
- II
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Robert's reagent solution is primarily used in the classical Robert's test for detecting proteinuria, which is the presence of protein in urine. This test is crucial in clinical chemistry for diagnosing various conditions, including kidney disease, diabetes, and hypertension. Additionally, the reagent can be employed in educational settings for teaching purposes in biochemistry and clinical laboratory science. Its use extends to research laboratories where protein detection in biological samples is necessary.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1290F
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- N/A
| Test | Standard | Observed |
|---|---|---|
| Appearance | Clear, colorless liquid | Clear, colorless liquid |
| Density | 1.2 g/cm³ | 1.2 g/cm³ |
| pH | <1 | <1 |
| Boiling Point | 83°C | 83°C |
| Solubility | Fully miscible in water | Fully miscible in water |
The above product is expected to comply with Ready-to-use reagent solution grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Ready-to-use reagent solution | N/A | 83°C | N/A | N/A | N/A | Fully miscible in water |
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