Rose Bengal
- CAS Number
- 632-69-9
- HSN Code
- 32049000
- Molecular Formula
- C20H2Cl4I4Na2O5
- Catalogue Number
- AL01293
- Purity
- Min. 85%
- Molecular Weight
- 1017.64 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Rose Bengal (AR, C.I. No.45440) is a xanthene dye that is primarily utilized in the field of ophthalmology for its ability to detect damaged corneal cells. This compound is notable for its vivid dark red-brown color and its high purity, making it suitable for various biological staining applications. With a minimum dye content of 85%, it is classified as an analytical reagent (AR grade), ensuring its reliability in laboratory settings. The molecular structure of Rose Bengal includes multiple halogen atoms, contributing to its unique properties and applications in both medical and research environments.
Also known as C.I. No.45440, 4,5,6,7-Tetrachloro-2',4',5',7'-tetrabromo-fluorescein.
- Catalogue No.
- AL01293
- CAS Number
- 632-69-9
- Molecular Formula
- C20H2Cl4I4Na2O5
- Molecular Weight
- 1017.64 g/mol
- HSN Code
- 32049000
- Grade
- AR grade
- Product Form
- Substance
Rose Bengal appears as a dark red-brown powder, which is highly soluble in water, allowing for easy preparation of solutions for various applications. The compound has a melting point of approximately 220 °C, and its solubility in water is around 0.5 g/100 mL at room temperature. The density of Rose Bengal is approximately 1.5 g/cm³, and it exhibits a strong absorption in the visible spectrum, particularly around 540 nm, which is characteristic of xanthene dyes. Its stability under normal laboratory conditions makes it a preferred choice for biological staining.
- Appearance
- Dark red-brown powder
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g glass bottles
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Rose Bengal
- Catalogue No.
- AL01293
- Product form
- Substance
- CAS Number
- 632-69-9
- EC Number
- 211-942-9
- Grade
- AR grade
- EC Index Number
- Not applicable
- Chemical Formula
- C20H2Cl4I4Na2O5
- HSN Code
- 32049000
- Synonyms
- C.I. No.45440, 4,5,6,7-Tetrachloro-2',4',5',7'-tetrabromo-fluorescein
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Used as a biological stain and ophthalmic diagnostic dye.
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause skin and eye irritation.
3.1 — Substance
- Name
- Rose Bengal
- CAS No.
- 632-69-9
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of the skin, eyes, and respiratory tract.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes in a fire. Use appropriate fire-fighting measures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use absorbent material to clean up spills and dispose of in accordance with local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use personal protective equipment as required.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from light and incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use in a well-ventilated area. If exposure limits are exceeded, use appropriate respiratory protection.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red-brown
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 220 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.5 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; LD50 (oral, rat) > 5000 mg/kg.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- Not a skin sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Rose Bengal is extensively used in ophthalmology as a diagnostic dye to identify damaged or dead corneal and conjunctival cells, aiding in the assessment of ocular surface health. Beyond its medical applications, it serves as a general biological stain in histology and microbiology, allowing researchers to visualize cellular structures and processes. Additionally, Rose Bengal is employed in photodynamic therapy due to its ability to generate reactive oxygen species upon light activation, making it useful in cancer treatment research. Its applications extend to environmental studies, where it is used as a tracer dye in hydrological studies to track water movement and sediment transport.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01293
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 1017.64 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark red powder | Dark red powder |
| Absorption maximum max (0.001%; in water + 1ml 1% Na2CO3 Solution) | 544 - 550nm | 547 nm |
| Absorptivity (A1%/1cm in water + 1ml 1% Na2CO3 Solution at max) | Min. 780 | 796 |
| Loss on drying (110°C) | Max. 10% | 6.1% |
The above product complies with AR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| AR grade | Min. 85% | 220 °C | Max. 1% | Max. 0.5% | None detected | Soluble in water |
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