Tris(hydroxymethyl)aminomethane hydrochloride
- CAS Number
- 1185-53-1
- HSN Code
- 29225090
- Molecular Formula
- C4H11ClN2O3
- Catalogue Number
- AL1544A
- Purity
- 99%
- Molecular Weight
- 157.60 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Tris-hydrochloride (AR, for molecular biology) is the acidic salt form of Tris buffer, essential for preparing precisely pH-adjusted Tris buffers. It is widely used in molecular biology applications due to its ability to maintain a stable pH environment, which is crucial for various biochemical reactions. This compound is particularly valued for its low absorbance in the UV range, making it suitable for spectrophotometric applications. The high purity level of 99% ensures minimal interference in sensitive experiments, making it a preferred choice for researchers.
Also known as Tris-HCl, Tris hydrochloride.
- Catalogue No.
- AL1544A
- CAS Number
- 1185-53-1
- Molecular Formula
- C4H11ClN2O3
- Molecular Weight
- 157.60 g/mol
- HSN Code
- 29225090
- Grade
- AR, For Molecular Biology
- Product Form
- Substance
Tris-hydrochloride appears as a white crystalline powder. It is highly soluble in water, with a solubility of approximately 1 g/mL at room temperature. The melting point of Tris-hydrochloride is around 170 °C, and it has a pH of approximately 2.8 when dissolved in water at a concentration of 1 M. The compound has a density of about 1.2 g/cm³. It is odorless and stable under normal conditions, making it easy to handle in laboratory settings.
- Appearance
- White crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 100 g, 500 g, and 1 kg bottles
- Storage
- Store in a cool, dry place, away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- Tris(hydroxymethyl)aminomethane hydrochloride
- Catalogue No.
- AL1544A
- Product form
- Substance
- CAS Number
- 1185-53-1
- EC Number
- 214-684-5
- Grade
- AR, For Molecular Biology
- EC Index Number
- Not applicable
- Chemical Formula
- C4H11ClN2O3
- HSN Code
- 29225090
- Synonyms
- Tris-HCl, Tris hydrochloride
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Buffering agent in molecular biology
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None known
3.1 — Substance
- Name
- Tris(hydroxymethyl)aminomethane hydrochloride
- CAS No.
- 1185-53-1
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin, eyes, and respiratory tract.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Non-flammable. Use appropriate extinguishing media for surrounding materials.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Sweep up and place in a suitable container for disposal.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, tightly closed.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal conditions; use in well-ventilated areas.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- White
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- 2.8 (1 M solution)
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 170 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.2 g/cm³
- Solubility
- Soluble in water (1 g/mL)
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Not classified as acutely toxic.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not applicable
- UN-No. (IMDG)
- Not applicable
- UN-No. (IATA)
- Not applicable
- UN-No. (ADN)
- Not applicable
- UN-No. (RID)
- Not applicable
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Tris-hydrochloride is primarily used in molecular biology for the preparation of Tris-HCl buffer solutions, which are essential for various applications including DNA and RNA electrophoresis, protein purification, and enzyme assays. It is also utilized in cell culture media and as a component in various biochemical assays. The compound's ability to maintain a stable pH makes it ideal for experiments that require precise pH control, such as PCR (Polymerase Chain Reaction) and other nucleic acid amplification techniques.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1544A
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 157.60 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | White crystalline powder | White crystalline powder |
| Assay | (Min.) 99.0% | 99.7% |
| pH (0.5M in water, at 20°C | 3.5 - 5.0 | 4.1 |
| Loss on drying at 110°C | <0.2% | <0.2% |
| Residue on ignition (900°C | <0.2% | Passes test |
| Solubility (0.5M in water, at 20°C) | Passes test | Passes test |
| Insoluble matter | Passes test | 0.006% |
| Maximum absorption (0.5M in water) | 0.015 | 0.008% |
| a) 260nm | 0.010 | |
| b) 280nm |
The above product complies with AR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| AR, For Molecular Biology | 99% | 170 °C | <0.5% | <0.1% | None detected | Soluble in water |
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