W.B.C. Diluting Fluid (Turck's) Extra Pure
- CAS Number
- -
- HSN Code
- 38229090
- Molecular Formula
- C2H4O2 / C25H30ClN3O4S
- Catalogue Number
- AL01573
- Purity
- ≥ 99%
- Molecular Weight
- 60.05 g/mol (Acetic acid) / 493.04 g/mol (Gentian violet)
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
W.B.C. diluting fluid (Turck's) extra pure is a specialized hematology reagent designed for the dilution and lysis of red blood cells, facilitating accurate white blood cell counting. This reagent is particularly notable for its extra pure grade, ensuring minimal interference in hematological analyses. The formulation consists of a solution of acetic acid and gentian violet, which not only aids in cell lysis but also provides a contrasting color for better visibility under a microscope. This product is essential in clinical laboratories for precise white blood cell enumeration, a critical parameter in various diagnostic procedures.
Also known as Acetic acid/gentian violet solution.
- Catalogue No.
- AL01573
- CAS Number
- -
- Molecular Formula
- C2H4O2 / C25H30ClN3O4S
- Molecular Weight
- 60.05 g/mol (Acetic acid) / 493.04 g/mol (Gentian violet)
- HSN Code
- 38229090
- Grade
- Extra Pure
- Product Form
- Mixture
W.B.C. diluting fluid appears as a pale violet liquid, indicative of the presence of gentian violet. The solution has a density of approximately 1.01 g/cm³, and the acetic acid component has a boiling point of 118.1 °C and a melting point of 16.6 °C. The solution is miscible with water, allowing for easy preparation and use in laboratory settings. The pH of the solution is typically around 4.75, reflecting the acidic nature of acetic acid. The product is stable under normal laboratory conditions and should be stored in a cool, dry place away from direct sunlight.
- Appearance
- Pale violet liquid
- Shelf Life
- 12 months
- Packaging
- Available in 500 mL bottles
- Storage
- Store in a cool, dry place, away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- W.B.C. Diluting Fluid (Turck's) Extra Pure
- Catalogue No.
- AL01573
- Product form
- Mixture
- CAS Number
- Not applicable
- EC Number
- Not applicable
- Grade
- Extra Pure
- EC Index Number
- Not applicable
- Chemical Formula
- C2H4O2 / C25H30ClN3O4S
- HSN Code
- 38229090
- Synonyms
- Acetic acid/gentian violet solution
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Hematology reagent for white blood cell counting
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Mildly corrosive due to acetic acid content
3.1 — Substance
- Name
- W.B.C. Diluting Fluid (Turck's) Extra Pure
- CAS No.
- Not applicable
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Seek medical attention if irritation persists.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; nausea if ingested.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Combustible material; may produce toxic fumes in a fire.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Clean up spills immediately to prevent slipping hazards.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Use gloves and safety goggles when handling. Ensure adequate ventilation in the work area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use in a well-ventilated area; if necessary, use a respirator suitable for organic vapors.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Pale violet
- Odour
- Vinegar-like (acetic acid)
- Odour threshold
- Not determined
- pH value
- 4.75
- Relative evaporation rate (butylacetate=1)
- <1
- Melting point
- 16.6 °C (Acetic acid)
- Freezing point
- Not determined
- Boiling point
- 118.1 °C (Acetic acid)
- Flash point
- 39 °C (Acetic acid)
- Auto-ignition temperature
- Not determined
- Decomposition temperature
- Not determined
- Flammability (solid, gas)
- Not applicable
- Vapour pressure
- 11.9 mmHg at 20 °C (Acetic acid)
- Relative vapour density at 20°C
- 1.07 (Acetic acid)
- Density
- 1.01 g/cm³
- Solubility
- Miscible with water
- Log Pow
- -0.31 (Acetic acid)
- Viscosity, kinematic
- Not determined
- Viscosity, dynamic
- Not determined
- Explosive properties
- Not applicable
- Oxidising properties
- Not applicable
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizers, strong bases, and reactive metals.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Acetic acid: LD50 (oral, rat) 3310 mg/kg; Gentian violet: LD50 (oral, rat) 2000 mg/kg.
- Skin corrosion/irritation
- Mildly irritating to skin.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- May cause allergic skin reactions in sensitive individuals.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- May cause respiratory irritation.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
This diluting fluid is primarily used in manual white blood cell counting, a fundamental procedure in clinical hematology. It is employed in conjunction with a hemocytometer, allowing for the accurate assessment of white blood cell concentrations in blood samples. The reagent is also utilized in various hematological research applications, including studies on blood disorders and immune responses. Additionally, the fluid's properties make it suitable for educational purposes in teaching laboratories, where students learn about blood cell morphology and counting techniques.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01573
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 60.05 g/mol (Acetic acid) / 493.04 g/mol (Gentian violet)
| Test | Standard | Observed |
|---|---|---|
| Assay (Purity) | ≥ 99% | 99.5% |
| pH Value | 4.5 - 5.5 | 4.75 |
| Density | 1.00 - 1.02 g/cm³ | 1.01 g/cm³ |
| Moisture Content | ≤ 1% | 0.5% |
| Residue on Ignition | ≤ 0.1% | 0.05% |
| Related Substances | None detected | None detected |
The above product is expected to comply with Extra Pure grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Extra Pure | ≥ 99% | 118.1 °C (Acetic acid) | ≤ 1% | ≤ 0.1% | None detected | Completely miscible with water |
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