2,4-Dinitrophenylhydrazine solution

Ready-to-use reagent solution For Laboratory Use
CAS Number
-
HSN Code
38229090
Molecular Formula
C10H10N4O4
Catalogue Number
AL01947
Purity
≥ 98%
Molecular Weight
238.21 g/mol

Complies with : IP | BP | USP | EP | ACS

ISO 9001 ISO 14001 GMP COA with every batch

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Key Properties

Appearance Clear orange-red liquid
Shelf Life 12 months
Packaging Available in 100 mL and 500 mL bottles
Storage Store in a cool, dry place away from light and incompatible materials.

Brady's reagent (2,4-dinitrophenylhydrazine solution) is a classic qualitative test reagent used to detect aldehydes and ketones via a colored precipitate. It is a ready-to-use reagent solution that provides a simple method for identifying carbonyl compounds in various samples.

Also known as Brady's reagent, 2,4-DNPH solution.

Catalogue No.
AL01947
CAS Number
-
Molecular Formula
C10H10N4O4
Molecular Weight
238.21 g/mol
HSN Code
38229090
Grade
Ready-to-use reagent solution
Product Form
Mixture

Clear orange-red liquid with a density of 1.06 g/mL. The solution is stable under recommended storage conditions and should be handled with care due to its chemical properties.

Appearance
Clear orange-red liquid
Shelf Life
12 months
Packaging
Available in 100 mL and 500 mL bottles
Storage
Store in a cool, dry place away from light and incompatible materials.

1.1 — Product identifier

Chemical Name
2,4-Dinitrophenylhydrazine solution
Catalogue No.
AL01947
Product form
Mixture
CAS Number
Not applicable
EC Number
204-450-0
Grade
Ready-to-use reagent solution
EC Index Number
607-115-00-0
Chemical Formula
C10H10N4O4
HSN Code
38229090
Synonyms
Brady's reagent, 2,4-DNPH solution

1.2 — Relevant identified uses of the substance or mixture and uses advised against

Use of the substance/mixture
Qualitative detection of aldehydes and ketones
Uses advised against
Not specified

1.3 — Details of the supplier of the safety data sheet

Company identification
Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.

1.4 — Emergency Contact Details

Phone / e-Mail
Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in

2.3 — Other hazards

Other hazards
Potentially explosive when dry; handle with care.

3.1 — Substance

Name
2,4-Dinitrophenylhydrazine solution
CAS No.
Not applicable

4.1 — Description of first aid measures

First-aid measures after inhalation
Move to fresh air. If symptoms persist, seek medical attention.
First-aid measures after skin contact
Wash with soap and water. If irritation occurs, seek medical attention.
First-aid measures after eye contact
Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical advice.
First-aid measures after ingestion
Do not induce vomiting. Rinse mouth and seek medical attention.

4.2 — Most important symptoms and effects, both acute and delayed

Symptoms relating to use
Irritation of eyes, skin, and respiratory tract; potential allergic reactions.

4.3 — Indication of any immediate medical attention and special treatment needed

General information
Treat symptomatically. Show this safety data sheet to the treating physician.

5.1 — Extinguishing media

Suitable extinguishing media
Water spray, carbon dioxide, dry chemical powder, foam.
Unsuitable extinguishing media
Not specified

5.2 — Special hazards arising from the substance or mixture

Special procedures
May emit toxic fumes in a fire. Use water spray to cool containers.

5.3 — Advice for firefighters

Protection during firefighting
Do not enter fire area without proper protective equipment, including respiratory protection.

6.1.1 — For non-emergency personnel

Emergency procedures
Contain spill and absorb with inert material. Dispose of in accordance with local regulations.

6.3 — Methods and material for containment and cleaning up

Methods for cleaning up
Use appropriate protective equipment. Clean up spills immediately and dispose of waste properly.

6.4 — Reference to other sections

Reference
Not specified

7.1 — Precautions for safe handling

Precautions for safe handling
Use gloves and eye protection. Avoid contact with skin and eyes. Do not allow to dry out.
Hygiene measures
Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.

7.2 — Conditions for safe storage, including any incompatibilities

Storage conditions
Store in a cool, dry place, away from heat sources and incompatible materials.

8.2 — Exposure controls

Hand protection
Protective gloves (e.g. nitrile rubber)
Eye protection
Chemical safety goggles or safety glasses
Skin and body protection
Wear suitable protective clothing
Respiratory protection
Use in a well-ventilated area. If exposure limits are exceeded, use appropriate respiratory protection.

9.1 — Information on basic physical and chemical properties

Physical state
Liquid
Colour
Orange-red
Odour
Slightly aromatic
Odour threshold
No data available
pH value
Acidic
Relative evaporation rate (butylacetate=1)
No data available
Melting point
No data available
Freezing point
No data available
Boiling point
No data available
Flash point
No data available
Auto-ignition temperature
No data available
Decomposition temperature
No data available
Flammability (solid, gas)
Not flammable
Vapour pressure
No data available
Relative vapour density at 20°C
No data available
Density
1.06 g/mL
Solubility
Soluble in alcohol and water
Log Pow
No data available
Viscosity, kinematic
No data available
Viscosity, dynamic
No data available
Explosive properties
Potentially explosive when dry
Oxidising properties
No data available
Explosive limits
No data available

10.1 — Reactivity

Reactivity
Stable under normal conditions. Avoid contact with strong oxidizers.

10.2 — Chemical stability

Chemical stability
Stable under recommended storage conditions.

10.3 — Possibility of hazardous reactions

Possibility of hazardous reactions
No dangerous reactions known under normal conditions of use.

10.4 — Conditions to avoid

Conditions to avoid
Avoid contact with incompatible materials and excessive heat.

10.5 — Incompatible materials

Incompatible materials
Strong oxidizing agents, strong acids.

10.6 — Hazardous decomposition products

Hazardous decomposition products
Not specified

11.1 — Information on toxicological effects

Acute toxicity
Harmful if swallowed or inhaled. May cause skin and eye irritation.
Skin corrosion/irritation
Causes skin irritation.
Serious eye damage/irritation
Causes serious eye irritation.
Respiratory or skin sensitisation
May cause allergic skin reaction.
Germ cell mutagenicity
Not classified
Carcinogenicity
Not classified
Specific target organ toxicity (single exposure)
No data available
Specific target organ toxicity (repeated exposure)
Not classified

12.1 — Toxicity

Toxicity information
Not specified

12.2 — Persistence - degradability

Persistence - degradability
Not specified

12.3 — Bioaccumulative potential

Bioaccumulative potential
Not specified

12.4 — Mobility in soil

Mobility in soil
Not specified

12.6 — Other adverse effects

Environmental precautions
Not specified

13.1 — Waste treatment methods

Additional Info
Dispose of contents/container in accordance with local, regional, national and international regulations.

14.1 — UN number

UN-No. (ADR)
Not assigned
UN-No. (IMDG)
Not assigned
UN-No. (IATA)
Not assigned
UN-No. (ADN)
Not assigned
UN-No. (RID)
Not assigned

14.2 — UN proper shipping name

Proper Shipping Name (ADR)
Not classified as dangerous goods
Proper Shipping Name (IMDG)
Not classified as dangerous goods
Proper Shipping Name (IATA)
Not classified as dangerous goods
Proper Shipping Name (ADN)
Not classified as dangerous goods
Proper Shipping Name (RID)
Not classified as dangerous goods

14.3 — Transport hazard class(es)

Transport hazard class(es)
Not applicable

14.4 — Packing group

Packing group (ADR)
Not applicable
Packing group (IMDG)
Not applicable
Packing group (IATA)
Not applicable
Packing group (ADN)
Not applicable
Packing group (RID)
Not applicable

14.5 — Environmental hazards

Dangerous for the environment
Not specified
Marine pollutant
Not specified
Other information
No supplementary information available

15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture

15.1.1 EU-Regulations
Not specified

Brady's reagent is primarily used in organic chemistry laboratories for the qualitative detection of aldehyde and ketone functional groups. The formation of a colored hydrazone precipitate indicates the presence of these carbonyl compounds.

ISO 9001 ISO 14001 GMP COA with every batch
Batch Number
AL-2026-AL01947
Manufacturing Date
September-2026
Expiry Date
September-2031
Molecular Weight
238.21 g/mol
Test Standard Observed
Assay (Purity) ≥ 98% 99.5%
Moisture Content ≤ 5% 3.2%
Residue on Ignition ≤ 0.5% 0.2%
Related Substances None detected None detected
Solubility Soluble in water and alcohol Fully soluble in both solvents

The above product is expected to comply with Ready-to-use reagent solution grade specification.

Technical Data Sheet

Grade Assay/Purity Melting/Boiling Point Moisture/LOD Residue on Ignition Related Substances Solubility
Ready-to-use reagent solution ≥ 98% No data available ≤ 5% ≤ 0.5% None detected Soluble in water and alcohol
View full Technical Data Sheet

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