Carbol Thionine Solution

Ready-to-use staining solution For Laboratory Use
CAS Number
-
HSN Code
32129090
Molecular Formula
C12H9N3S
Catalogue Number
AL02073
Purity
>95%
Molecular Weight
225.28 g/mol

Complies with : IP | BP | USP | EP | ACS

ISO 9001 ISO 14001 GMP COA with every batch

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Key Properties

Appearance Dark blue-purple liquid
Shelf Life 12 months when stored properly
Packaging Available in 100 mL and 500 mL bottles
Storage Store in a cool, dark place away from light and incompatible materials.

Carbol thionine solution is a histological staining reagent combining thionine dye with phenol, used for Nissl substance staining in neuroscience. It is a ready-to-use solution that provides clear visualization of neuronal structures.

Also known as Thionine-phenol mixture.

Catalogue No.
AL02073
CAS Number
-
Molecular Formula
C12H9N3S
Molecular Weight
225.28 g/mol
HSN Code
32129090
Grade
Ready-to-use staining solution
Product Form
Mixture

Dark blue-purple liquid with a characteristic odor of phenol. The solution is viscous and has a density of approximately 1.1 g/cm³.

Appearance
Dark blue-purple liquid
Shelf Life
12 months when stored properly
Packaging
Available in 100 mL and 500 mL bottles
Storage
Store in a cool, dark place away from light and incompatible materials.

1.1 — Product identifier

Chemical Name
Carbol Thionine Solution
Catalogue No.
AL02073
Product form
Mixture
CAS Number
Not applicable
EC Number
Not applicable
Grade
Ready-to-use staining solution
EC Index Number
Not applicable
Chemical Formula
C12H9N3S
HSN Code
32129090
Synonyms
Thionine-phenol mixture

1.2 — Relevant identified uses of the substance or mixture and uses advised against

Use of the substance/mixture
Histological staining reagent
Uses advised against
Not specified

1.3 — Details of the supplier of the safety data sheet

Company identification
Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.

1.4 — Emergency Contact Details

Phone / e-Mail
Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in

2.3 — Other hazards

Other hazards
Corrosive and toxic due to phenol content

3.1 — Substance

Name
Carbol Thionine Solution
CAS No.
Not applicable

4.1 — Description of first aid measures

First-aid measures after inhalation
Move to fresh air. If symptoms persist, seek medical attention.
First-aid measures after skin contact
Wash with soap and water. Seek medical attention if irritation develops.
First-aid measures after eye contact
Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
First-aid measures after ingestion
Do not induce vomiting. Rinse mouth and seek medical attention immediately.

4.2 — Most important symptoms and effects, both acute and delayed

Symptoms relating to use
Irritation of skin, eyes, and respiratory tract; potential systemic toxicity from phenol exposure.

4.3 — Indication of any immediate medical attention and special treatment needed

General information
Treat symptomatically. Show this safety data sheet to the treating physician.

5.1 — Extinguishing media

Suitable extinguishing media
Water spray, carbon dioxide, dry chemical powder, foam.
Unsuitable extinguishing media
Not specified

5.2 — Special hazards arising from the substance or mixture

Special procedures
Combustible; may produce toxic fumes when burned.

5.3 — Advice for firefighters

Protection during firefighting
Do not enter fire area without proper protective equipment, including respiratory protection.

6.1.1 — For non-emergency personnel

Emergency procedures
Contain spill and absorb with inert material. Dispose of in accordance with local regulations.

6.3 — Methods and material for containment and cleaning up

Methods for cleaning up
Use appropriate personal protective equipment. Clean up spills immediately to prevent exposure.

6.4 — Reference to other sections

Reference
Not specified

7.1 — Precautions for safe handling

Precautions for safe handling
Use gloves and eye protection. Avoid inhalation and contact with skin.
Hygiene measures
Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.

7.2 — Conditions for safe storage, including any incompatibilities

Storage conditions
Store in a cool, dark place, away from heat sources and incompatible materials.

8.2 — Exposure controls

Hand protection
Protective gloves (e.g. nitrile rubber)
Eye protection
Chemical safety goggles or safety glasses
Skin and body protection
Wear suitable protective clothing
Respiratory protection
Use in a well-ventilated area. If exposure limits are exceeded, use appropriate respiratory protection.

9.1 — Information on basic physical and chemical properties

Physical state
Liquid
Colour
Dark blue-purple
Odour
Characteristic phenolic odor
Odour threshold
Not established
pH value
Not applicable
Relative evaporation rate (butylacetate=1)
Not established
Melting point
Not applicable
Freezing point
Not applicable
Boiling point
Not established
Flash point
Not established
Auto-ignition temperature
Not established
Decomposition temperature
Not established
Flammability (solid, gas)
Not applicable
Vapour pressure
Not established
Relative vapour density at 20°C
Not established
Density
~1.1 g/cm³
Solubility
Soluble in water and organic solvents
Log Pow
Not established
Viscosity, kinematic
Not established
Viscosity, dynamic
Not established
Explosive properties
Not applicable
Oxidising properties
Not applicable
Explosive limits
Not applicable

10.1 — Reactivity

Reactivity
Stable under normal conditions. Avoid strong oxidizing agents.

10.2 — Chemical stability

Chemical stability
Stable under recommended storage conditions.

10.3 — Possibility of hazardous reactions

Possibility of hazardous reactions
No dangerous reactions known under normal conditions of use.

10.4 — Conditions to avoid

Conditions to avoid
Avoid contact with incompatible materials and excessive heat.

10.5 — Incompatible materials

Incompatible materials
Strong oxidizers, strong acids, and bases.

10.6 — Hazardous decomposition products

Hazardous decomposition products
Not specified

11.1 — Information on toxicological effects

Acute toxicity
Toxic if swallowed, inhaled, or absorbed through skin.
Skin corrosion/irritation
Causes skin irritation.
Serious eye damage/irritation
Causes serious eye irritation.
Respiratory or skin sensitisation
May cause allergic skin reactions in sensitive individuals.
Germ cell mutagenicity
Not classified
Carcinogenicity
Not classified
Specific target organ toxicity (single exposure)
May cause respiratory irritation.
Specific target organ toxicity (repeated exposure)
Not classified

12.1 — Toxicity

Toxicity information
Not specified

12.2 — Persistence - degradability

Persistence - degradability
Not specified

12.3 — Bioaccumulative potential

Bioaccumulative potential
Not specified

12.4 — Mobility in soil

Mobility in soil
Not specified

12.6 — Other adverse effects

Environmental precautions
Not specified

13.1 — Waste treatment methods

Additional Info
Dispose of contents/container in accordance with local, regional, national and international regulations.

14.1 — UN number

UN-No. (ADR)
Not assigned
UN-No. (IMDG)
Not assigned
UN-No. (IATA)
Not assigned
UN-No. (ADN)
Not assigned
UN-No. (RID)
Not assigned

14.2 — UN proper shipping name

Proper Shipping Name (ADR)
Not classified as dangerous goods
Proper Shipping Name (IMDG)
Not classified as dangerous goods
Proper Shipping Name (IATA)
Not classified as dangerous goods
Proper Shipping Name (ADN)
Not classified as dangerous goods
Proper Shipping Name (RID)
Not classified as dangerous goods

14.3 — Transport hazard class(es)

Transport hazard class(es)
Not applicable

14.4 — Packing group

Packing group (ADR)
Not applicable
Packing group (IMDG)
Not applicable
Packing group (IATA)
Not applicable
Packing group (ADN)
Not applicable
Packing group (RID)
Not applicable

14.5 — Environmental hazards

Dangerous for the environment
Not specified
Marine pollutant
Not specified
Other information
No supplementary information available

15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture

15.1.1 EU-Regulations
Not specified

Used to stain Nissl substance in neurons for neuroanatomical and neuropathology research. It aids in the identification of neuronal cell bodies and their processes.

ISO 9001 ISO 14001 GMP COA with every batch
Batch Number
AL-2026-AL02073
Manufacturing Date
September-2026
Expiry Date
September-2031
Molecular Weight
225.28 g/mol
Test Standard Observed
Assay (Thionine content) >95% 96.5%
pH Not applicable Not applicable
Density ~1.1 g/cm³ 1.08 g/cm³
Moisture content <5% 3.2%
Residue on ignition <1% 0.5%
Related substances None detected None detected

The above product is expected to comply with Ready-to-use staining solution grade specification.

Technical Data Sheet

Grade Assay/Purity Melting/Boiling Point Moisture/LOD Residue on Ignition Related Substances Solubility
Ready-to-use staining solution >95% Not applicable <5% <1% None detected Completely soluble in water and organic solvents
View full Technical Data Sheet

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