Ethidium bromide
- CAS Number
- 1239-45-8
- HSN Code
- 29339900
- Molecular Formula
- C21H20BrN3
- Catalogue Number
- AL0675B
- Purity
- ≥ 98%
- Molecular Weight
- 394.31 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Ethidium bromide (AR grade) is one of the most widely used intercalating dyes for visualizing DNA/RNA in agarose gels under UV light. It is known for its ability to intercalate between base pairs of nucleic acids, allowing for effective visualization during electrophoresis.
Also known as Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide.
- Catalogue No.
- AL0675B
- CAS Number
- 1239-45-8
- Molecular Formula
- C21H20BrN3
- Molecular Weight
- 394.31 g/mol
- HSN Code
- 29339900
- Grade
- AR grade
- Product Form
- Substance
Dark red-purple crystalline powder; soluble in water. It has a melting point of approximately 110-112 °C and a boiling point of 250 °C.
- Appearance
- Dark red-purple crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 10 g and 100 g bottles
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Ethidium bromide
- Catalogue No.
- AL0675B
- Product form
- Substance
- CAS Number
- 1239-45-8
- EC Number
- 214-200-4
- Grade
- AR grade
- EC Index Number
- 611-007-00-2
- Chemical Formula
- C21H20BrN3
- HSN Code
- 29339900
- Synonyms
- Ethidium bromide, 1,3-bis(4-(dimethylamino)phenyl)isoquinolinium bromide
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Laboratory reagent for DNA/RNA visualization
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Mutagenic and suspected carcinogen; handle with care.
3.1 — Substance
- Name
- Ethidium bromide
- CAS No.
- 1239-45-8
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. Seek medical attention if irritation develops.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Do not induce vomiting. Rinse mouth and seek medical attention immediately.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Symptoms of exposure may include irritation of the respiratory tract, skin, and eyes; nausea; and potential mutagenic effects.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Toxic fumes may be released in a fire. Use water spray to cool containers.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Evacuate area and ventilate. Wear appropriate protective equipment. Contain spill and collect for disposal.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Absorb spill with inert material and dispose of as hazardous waste.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use appropriate personal protective equipment (PPE) including gloves and goggles.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a tightly closed container in a cool, dry, well-ventilated area away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a NIOSH-approved respirator if exposure limits are exceeded or if irritation occurs.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red-purple
- Odour
- Odourless
- Odour threshold
- No data available
- pH value
- No data available
- Relative evaporation rate (butylacetate=1)
- No data available
- Melting point
- 110-112 °C
- Freezing point
- No data available
- Boiling point
- 250 °C
- Flash point
- No data available
- Auto-ignition temperature
- No data available
- Decomposition temperature
- No data available
- Flammability (solid, gas)
- No
- Vapour pressure
- No data available
- Relative vapour density at 20°C
- No data available
- Density
- 1.5 g/cm³
- Solubility
- Soluble in water
- Log Pow
- No data available
- Viscosity, kinematic
- No data available
- Viscosity, dynamic
- No data available
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- No data available
10.1 — Reactivity
- Reactivity
- Stable under recommended storage conditions. Avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids, and bases.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- LD50 (oral, rat) = 10 mg/kg
- Skin corrosion/irritation
- Causes skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- May cause sensitization by skin contact.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- May cause respiratory irritation.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- 2811
- UN-No. (IMDG)
- 2811
- UN-No. (IATA)
- 2811
- UN-No. (ADN)
- 2811
- UN-No. (RID)
- 2811
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (IMDG)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (IATA)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (ADN)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
- Proper Shipping Name (RID)
- Toxic solid, organic, n.o.s. (Ethidium bromide)
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 6.1
14.4 — Packing group
- Packing group (ADR)
- I
- Packing group (IMDG)
- I
- Packing group (IATA)
- I
- Packing group (ADN)
- I
- Packing group (RID)
- I
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Used as the classic intercalating fluorescent stain for visualizing DNA/RNA bands in agarose gel electrophoresis. It is also utilized in various molecular biology applications, including nucleic acid quantification and detection.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL0675B
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 394.31 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark red crystalline powder | Dark red crystalline powder |
| Assay | Min. 98.0% | 98.74% |
| Absorption maximum (In Methanol) | 524-527 nm | 527 nm |
| Absorptivity (A1%/1 cm, in Methanol on dried substance) | 155-165 | 158 |
| Loss on drying (130°C/4 hours) | < 7 % |
The above product complies with AR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| AR grade | ≥ 98% | 110-112 °C | ≤ 0.5% | ≤ 0.1% | None detected | Soluble in water |
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