Ethidium bromide
- CAS Number
- 1239-45-8
- HSN Code
- 29339990
- Molecular Formula
- C21H20BrN3
- Catalogue Number
- AL0675C
- Purity
- ~1%
- Molecular Weight
- 394.31 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Ethidium bromide solution (~1% in water) is a ready-made preparation, saving the step of handling the solid dye powder directly.
Also known as Ethidium bromide solution.
- Catalogue No.
- AL0675C
- CAS Number
- 1239-45-8
- Molecular Formula
- C21H20BrN3
- Molecular Weight
- 394.31 g/mol
- HSN Code
- 29339990
- Grade
- ~1% in water
- Product Form
- Mixture
Dark red-purple liquid, density 1.00 g/mL.
- Appearance
- Dark red-purple liquid
- Shelf Life
- 12 months
- Packaging
- 500 mL amber glass bottle
- Storage
- Store at 2-8°C in a cool, dark place.
1.1 — Product identifier
- Chemical Name
- Ethidium bromide
- Catalogue No.
- AL0675C
- Product form
- Mixture
- CAS Number
- 1239-45-8
- EC Number
- 214-200-4
- Grade
- ~1% in water
- EC Index Number
- 612-080-00-0
- Chemical Formula
- C21H20BrN3
- HSN Code
- 29339990
- Synonyms
- Ethidium bromide solution
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Laboratory reagent for DNA/RNA visualization
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Mutagenic and suspected carcinogen; handle with care.
3.1 — Substance
- Name
- Ethidium bromide
- CAS No.
- 1239-45-8
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. Seek medical attention if irritation develops.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Do not induce vomiting. Rinse mouth and seek medical attention immediately.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Nausea, vomiting, skin irritation, eye irritation.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Toxic fumes may be released in a fire. Use water spray to cool containers.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Evacuate area and ventilate. Wear appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Contain spill with inert material and dispose of as hazardous waste.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Use personal protective equipment (PPE) including gloves and goggles. Avoid contact with skin and eyes.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dark place at 2-8°C.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use in a well-ventilated area. If exposure limits are exceeded, use appropriate respiratory protection.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Dark red-purple
- Odour
- Slightly aromatic
- Odour threshold
- No data available
- pH value
- No data available
- Relative evaporation rate (butylacetate=1)
- No data available
- Melting point
- No data available
- Freezing point
- No data available
- Boiling point
- No data available
- Flash point
- No data available
- Auto-ignition temperature
- No data available
- Decomposition temperature
- No data available
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- No data available
- Relative vapour density at 20°C
- No data available
- Density
- 1.00 g/mL
- Solubility
- Soluble in water
- Log Pow
- No data available
- Viscosity, kinematic
- No data available
- Viscosity, dynamic
- No data available
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- No data available
10.1 — Reactivity
- Reactivity
- Stable under normal conditions. Avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Toxic if swallowed, in contact with skin, or if inhaled.
- Skin corrosion/irritation
- Causes skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- May cause an allergic skin reaction.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- May cause damage to organs through prolonged or repeated exposure.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- 2810
- UN-No. (IMDG)
- 2810
- UN-No. (IATA)
- 2810
- UN-No. (ADN)
- 2810
- UN-No. (RID)
- 2810
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Ethidium bromide solution
- Proper Shipping Name (IMDG)
- Ethidium bromide solution
- Proper Shipping Name (IATA)
- Ethidium bromide solution
- Proper Shipping Name (ADN)
- Ethidium bromide solution
- Proper Shipping Name (RID)
- Ethidium bromide solution
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 6.1
14.4 — Packing group
- Packing group (ADR)
- III
- Packing group (IMDG)
- III
- Packing group (IATA)
- III
- Packing group (ADN)
- III
- Packing group (RID)
- III
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Used directly as a DNA/RNA intercalating stain for gel electrophoresis visualization under UV light.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL0675C
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 394.31 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay | ~1% | 1.02% |
| Density | 1.00 g/mL | 1.00 g/mL |
| Appearance | Dark red-purple liquid | Dark red-purple liquid |
| pH | Not applicable | Not applicable |
The above product is expected to comply with ~1% in water grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| ~1% in water | ~1% | No data available | No data available | No data available | No data available | Soluble in water |
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