Evan's Blue
- CAS Number
- 314-13-6
- HSN Code
- 32049000
- Molecular Formula
- C34H24N6Na4O14S4
- Catalogue Number
- AL00713
- Purity
- min. 85%
- Molecular Weight
- 960.81 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Evan's blue is a diazo dye widely used in biology to assess vascular permeability by binding to serum albumin. It is primarily utilized in microscopy applications due to its ability to provide clear visualization of vascular structures.
Also known as C.I. No.23860.
- Catalogue No.
- AL00713
- CAS Number
- 314-13-6
- Molecular Formula
- C34H24N6Na4O14S4
- Molecular Weight
- 960.81 g/mol
- HSN Code
- 32049000
- Grade
- For Microscopy
- Product Form
- Substance
Dark blue-black crystalline powder with a high purity grade suitable for microscopy. It has a minimum dye content of 85%.
- Appearance
- Dark blue-black crystalline powder
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place away from light.
1.1 — Product identifier
- Chemical Name
- Evan's Blue
- Catalogue No.
- AL00713
- Product form
- Substance
- CAS Number
- 314-13-6
- EC Number
- Not specified
- Grade
- For Microscopy
- EC Index Number
- Not specified
- Chemical Formula
- C34H24N6Na4O14S4
- HSN Code
- 32049000
- Synonyms
- C.I. No.23860
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Used as a vascular-permeability tracer dye and plasma-volume determination reagent.
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None identified.
3.1 — Substance
- Name
- Evan's Blue
- CAS No.
- 314-13-6
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin, eyes, and respiratory tract.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- No specific fire hazards identified; use standard fire-fighting procedures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate methods. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Clean up spills immediately to prevent slipping hazards.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if ventilation is inadequate.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark blue-black
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Not specified
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not specified
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- Not specified
- Solubility
- Soluble in water and alcohol
- Log Pow
- Not specified
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Not classified as acutely toxic.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Evan's blue is used as a vascular-permeability tracer dye in physiology research and as a plasma-volume determination reagent.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL00713
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 960.81 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark Brown Powder | Dark Brown Powder |
| Assay | Min 90.0% | 90.36% |
| Absorptivity (A 1%/cm 612 nm pH 7 on dried substance) | 630 - 680 | 650 |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| For Microscopy | min. 85% | Not specified | Not specified | Not specified | Not specified | Soluble in water and alcohol |
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