Gram's Iodine
- CAS Number
- 12298-68-9
- HSN Code
- 38229090
- Molecular Formula
- C6H4I2K2O2
- Catalogue Number
- AL02316
- Purity
- ≥ 99%
- Molecular Weight
- 354.37 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Gram's iodine is a mordant solution essential to the Gram staining procedure, fixing the crystal violet dye within Gram-positive bacterial cell walls. It is commonly used in microbiology for the identification and classification of bacteria.
Also known as Iodine/potassium iodide complex.
- Catalogue No.
- AL02316
- CAS Number
- 12298-68-9
- Molecular Formula
- C6H4I2K2O2
- Molecular Weight
- 354.37 g/mol
- HSN Code
- 38229090
- Grade
- For Microscopy
- Product Form
- Mixture
Dark brown crystalline solid or solution with a characteristic iodine odor. The solution is typically prepared in a concentration suitable for microscopy applications.
- Appearance
- Dark brown crystalline solid or solution
- Shelf Life
- 2 years
- Packaging
- Available in glass bottles or plastic containers, typically 100 mL or 500 mL
- Storage
- Store in a cool, dry place away from light.
1.1 — Product identifier
- Chemical Name
- Gram's Iodine
- Catalogue No.
- AL02316
- Product form
- Mixture
- CAS Number
- 12298-68-9
- EC Number
- 235-442-0
- Grade
- For Microscopy
- EC Index Number
- Iodine: 204-650-8; Potassium iodide: 204-650-8
- Chemical Formula
- C6H4I2K2O2
- HSN Code
- 38229090
- Synonyms
- Iodine/potassium iodide complex
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Mordant in Gram staining and other laboratory applications.
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause irritation to skin and eyes; harmful if ingested.
3.1 — Substance
- Name
- Gram's Iodine
- CAS No.
- 12298-68-9
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention immediately.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; potential for gastrointestinal distress if ingested.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Iodine vapors may be harmful; use appropriate fire extinguishing media.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Clean area thoroughly after spill.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from light and incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if ventilation is inadequate.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid or solution
- Colour
- Dark brown
- Odour
- Characteristic iodine odor
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- Approximately 1.5 g/cm³
- Solubility
- Soluble in water and alcohol
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Oxidizing agent
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; reacts with strong oxidizers.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Harmful if swallowed; may cause irritation to skin and eyes.
- Skin corrosion/irritation
- May cause skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- Not a skin sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Used as the mordant step in Gram staining, forming an insoluble complex with crystal violet inside Gram-positive bacteria. It is also utilized in various laboratory applications involving iodine staining.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL02316
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 354.37 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay | ≥ 99% | 99.5% |
| Moisture Content | ≤ 0.5% | 0.3% |
| Residue on Ignition | ≤ 0.1% | 0.05% |
| Related Substances | None detected | None detected |
| Solubility | Soluble in water and alcohol | Soluble in both |
The above product is expected to comply with For Microscopy grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| For Microscopy | ≥ 99% | Not applicable | ≤ 0.5% | ≤ 0.1% | None detected | Soluble in water and alcohol |
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