Methyl Violet (M.S.)
- CAS Number
- 8004-87-3
- HSN Code
- 32041341
- Molecular Formula
- C24H27N3·HCl
- Catalogue Number
- AL01034
- Purity
- Min. 75% dye content
- Molecular Weight
- 393.95 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Methyl violet (M.S.) is a classic triarylmethane dye used in histology, microbiology, and as a general biological stain. It is known for its vibrant color and is commonly utilized in various staining techniques.
Also known as Basic Violet 1, Crystal Violet, Gentian Violet.
- Catalogue No.
- AL01034
- CAS Number
- 8004-87-3
- Molecular Formula
- C24H27N3·HCl
- Molecular Weight
- 393.95 g/mol
- HSN Code
- 32041341
- Grade
- M.S., C.I. No.42535
- Product Form
- Substance
Dark green to bronze crystalline powder with a melting point of approximately 200-205 °C. It is soluble in water and alcohol, with a density of about 1.2 g/cm³.
- Appearance
- Dark green to bronze crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g glass bottles
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Methyl Violet (M.S.)
- Catalogue No.
- AL01034
- Product form
- Substance
- CAS Number
- 8004-87-3
- EC Number
- 232-007-0
- Grade
- M.S., C.I. No.42535
- EC Index Number
- 612-030-00-0
- Chemical Formula
- C24H27N3·HCl
- HSN Code
- 32041341
- Synonyms
- Basic Violet 1, Crystal Violet, Gentian Violet
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Biological stain, pH indicator, and dye in histology and microbiology.
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Suspected carcinogen; may cause respiratory irritation.
3.1 — Substance
- Name
- Methyl Violet (M.S.)
- CAS No.
- 8004-87-3
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; potential carcinogenic effects.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes in a fire. Use water spray to cool containers.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Sweep up and place in a suitable container for disposal.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area and wear appropriate protective equipment.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a dust mask or respirator if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark green to bronze
- Odour
- Slightly aromatic
- Odour threshold
- Not determined
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not determined
- Melting point
- 200-205 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not determined
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- ~1.2 g/cm³
- Solubility
- Soluble in water and alcohol
- Log Pow
- Not determined
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions. Avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- LD50 (oral, rat) > 2000 mg/kg.
- Skin corrosion/irritation
- May cause skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- Not a skin sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- 3077
- UN-No. (IMDG)
- 3077
- UN-No. (IATA)
- 3077
- UN-No. (ADN)
- 3077
- UN-No. (RID)
- 3077
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Environmentally hazardous substance, solid, n.o.s.
- Proper Shipping Name (IMDG)
- Environmentally hazardous substance, solid, n.o.s.
- Proper Shipping Name (IATA)
- Environmentally hazardous substance, solid, n.o.s.
- Proper Shipping Name (ADN)
- Environmentally hazardous substance, solid, n.o.s.
- Proper Shipping Name (RID)
- Environmentally hazardous substance, solid, n.o.s.
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 9
14.4 — Packing group
- Packing group (ADR)
- III
- Packing group (IMDG)
- III
- Packing group (IATA)
- III
- Packing group (ADN)
- III
- Packing group (RID)
- III
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Methyl violet is primarily used as a general biological stain and pH indicator dye in histology research. It is also employed in microbiological applications for staining bacteria and fungi.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01034
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 393.95 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark green crystalline powder | Dark green crystalline powder |
| Dye content | Min.75% | 76.2% |
| Absorption Maxima | 583 - 587nm | 587nm |
| Loss on drying at 110°C | <8% | 5.1% |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| M.S. | Min. 75% dye content | 200-205 °C | Max. 5% | Max. 1% | None detected | Soluble in water and alcohol |
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