Methylene Blue
- CAS Number
- -
- HSN Code
- 32129090
- Molecular Formula
- C16H18ClN3S
- Catalogue Number
- AL0745A
- Purity
- N/A
- Molecular Weight
- 319.85 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Field's stain 'A' solution is a ready-made liquid preparation used for rapid blood-film staining, particularly in the detection of malaria parasites. It contains a methylene blue-based dye in solution, providing effective staining for microscopic examination.
Also known as Methylene blue solution, Field's stain A.
- Catalogue No.
- AL0745A
- CAS Number
- -
- Molecular Formula
- C16H18ClN3S
- Molecular Weight
- 319.85 g/mol
- HSN Code
- 32129090
- Grade
- Ready-to-use staining solution
- Product Form
- Mixture
The solution is a dark blue-green liquid with a density of 0.89 g/mL. It is characterized by its vivid color, which aids in the visualization of cellular components under a microscope.
- Appearance
- Dark blue-green liquid
- Shelf Life
- 12 months
- Packaging
- Available in 100 mL bottles
- Storage
- Store in a cool, dry place away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- Methylene Blue
- Catalogue No.
- AL0745A
- Product form
- Mixture
- CAS Number
- Not applicable
- EC Number
- 200-015-1
- Grade
- Ready-to-use staining solution
- EC Index Number
- 611-004-00-0
- Chemical Formula
- C16H18ClN3S
- HSN Code
- 32129090
- Synonyms
- Methylene blue solution, Field's stain A
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Staining agent for microscopy
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause skin and eye irritation
3.1 — Substance
- Name
- Methylene Blue
- CAS No.
- Not applicable
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. Seek medical attention if symptoms persist.
- First-aid measures after skin contact
- Wash with soap and water. Seek medical attention if irritation develops.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of skin and eyes, respiratory discomfort if inhaled.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Non-flammable, but may emit toxic fumes in a fire.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate protective equipment. Clean area thoroughly after spill.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a tightly closed container in a cool, dry place.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if ventilation is inadequate.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Dark blue-green
- Odour
- Slightly aromatic
- Odour threshold
- Not determined
- pH value
- Not determined
- Relative evaporation rate (butylacetate=1)
- Not determined
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- Not determined
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not determined
- Flammability (solid, gas)
- Not applicable
- Vapour pressure
- Not determined
- Relative vapour density at 20°C
- Not determined
- Density
- 0.89 g/mL
- Solubility
- Soluble in water
- Log Pow
- Not determined
- Viscosity, kinematic
- Not determined
- Viscosity, dynamic
- Not determined
- Explosive properties
- Not applicable
- Oxidising properties
- Not applicable
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; may cause irritation.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- Not expected to be a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
This solution is primarily used as part A of the rapid Field's staining method for the detection of malaria parasites in blood films. It is essential for laboratory diagnostics in hematology and parasitology.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL0745A
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 319.85 g/mol
| Test | Standard | Observed |
|---|---|---|
| Appearance | Dark blue-green liquid | Dark blue-green liquid |
| Density | 0.89 g/mL | 0.89 g/mL |
| pH | Not applicable | Not applicable |
| Solubility | Soluble in water | Soluble in water |
The above product is expected to comply with Ready-to-use staining solution grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Ready-to-use staining solution | N/A | N/A | N/A | N/A | N/A | Soluble in water |
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