N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride
- CAS Number
- 637-01-4
- HSN Code
- 29215190
- Molecular Formula
- C10H18Cl2N2
- Catalogue Number
- AL1500C
- Purity
- 99%
- Molecular Weight
- 237.18 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride is a highly pure redox indicator dye, primarily utilized in biochemical assays, particularly in the testing of cytochrome oxidase enzyme activity. This compound is notable for its role in microbiological diagnostics, where it serves as a key reagent in the oxidase test, aiding in the identification of certain bacteria based on their enzymatic activity. With a high purity grade of 99%, it is suitable for sensitive applications in research and clinical laboratories. Its molecular structure, characterized by the presence of two methyl groups on each nitrogen atom, contributes to its unique chemical properties and reactivity.
Also known as TMPhD, Tetramethyl-p-phenylenediamine dihydrochloride.
- Catalogue No.
- AL1500C
- CAS Number
- 637-01-4
- Molecular Formula
- C10H18Cl2N2
- Molecular Weight
- 237.18 g/mol
- HSN Code
- 29215190
- Grade
- Extra Pure
- Product Form
- Substance
N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride appears as a white to pale gray crystalline powder. It has a melting point of approximately 170-175 °C and is soluble in water, making it easy to incorporate into various aqueous solutions for laboratory use. The compound has a density of about 1.1 g/cm³ and exhibits a characteristic odor that is faint and not overpowering. Its solubility in water allows for its effective use in biochemical assays, where it can easily interact with other reagents and substrates.
- Appearance
- White to pale gray crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 100 g and 500 g bottles
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride
- Catalogue No.
- AL1500C
- Product form
- Substance
- CAS Number
- 637-01-4
- EC Number
- 211-946-9
- Grade
- Extra Pure
- EC Index Number
- 612-100-00-0
- Chemical Formula
- C10H18Cl2N2
- HSN Code
- 29215190
- Synonyms
- TMPhD, Tetramethyl-p-phenylenediamine dihydrochloride
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Redox indicator dye for enzyme activity testing
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause skin and eye irritation.
3.1 — Substance
- Name
- N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride
- CAS No.
- 637-01-4
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of skin, eyes, and respiratory tract.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes under fire conditions.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use inert absorbent material and dispose of in accordance with local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if ventilation is inadequate.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- White to pale gray
- Odour
- Faint
- Odour threshold
- Not determined
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not determined
- Melting point
- 170-175 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not determined
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.1 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not determined
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; LD50 (oral, rat) > 2000 mg/kg.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
This compound is primarily used in the oxidase test, which is crucial for detecting cytochrome c oxidase activity in various bacterial species. It is widely employed in microbiology laboratories for the identification of pathogens, particularly in differentiating between oxidase-positive and oxidase-negative bacteria. Beyond its application in microbiological diagnostics, N,N,N',N'-Tetramethyl-p-phenylenediamine dihydrochloride is also utilized in biochemical research to study enzyme kinetics and metabolic pathways, making it a valuable tool in both clinical and research settings.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1500C
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 237.18 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay | 99% min | 99.5% |
| Melting Point | 170-175 °C | 172 °C |
| Moisture Content | <0.5% | 0.3% |
| Residue on Ignition | <0.1% | 0.05% |
| Related Substances | None detected | None detected |
| Solubility | Soluble in water | Fully soluble in water at room temperature. |
The above product is expected to comply with Extra Pure grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Extra Pure | 99% | 170-175 °C | <0.5% | <0.1% | None detected | Soluble in water |
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