Polysorbate 20
- CAS Number
- 9005-64-5
- HSN Code
- 34024200
- Molecular Formula
- C58H114O26
- Catalogue Number
- AL01551
- Purity
- ≥ 99%
- Molecular Weight
- 1227.72 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Tween 20 Extra Pure, also known as polysorbate 20, is a nonionic surfactant widely utilized in biochemistry and molecular biology. It is particularly valued for its mild detergent properties, making it suitable for various applications in laboratory settings. This surfactant is derived from sorbitol and fatty acids, and its structure allows it to effectively reduce surface tension, facilitating the solubilization of hydrophobic compounds. Tween 20 is recognized for its ability to stabilize emulsions and enhance the solubility of proteins and other biomolecules, which is crucial in many biochemical assays and formulations.
Also known as Tween 20, Polysorbate 20.
- Catalogue No.
- AL01551
- CAS Number
- 9005-64-5
- Molecular Formula
- C58H114O26
- Molecular Weight
- 1227.72 g/mol
- HSN Code
- 34024200
- Grade
- Extra Pure
- Product Form
- Substance
Tween 20 Extra Pure appears as an amber to pale yellow viscous liquid. It has a density of approximately 1.105 g/mL, indicating its relatively high viscosity compared to water. The substance is soluble in water, which is a key characteristic that enhances its utility in various biochemical applications. The melting point is not applicable as it is a liquid at room temperature, and it has a boiling point of around 100 °C under standard atmospheric conditions. The product is odorless, making it suitable for sensitive applications where odor may interfere with results.
- Appearance
- Amber to pale yellow viscous liquid
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- Polysorbate 20
- Catalogue No.
- AL01551
- Product form
- Substance
- CAS Number
- 9005-64-5
- EC Number
- 500-018-3
- Grade
- Extra Pure
- EC Index Number
- Not specified
- Chemical Formula
- C58H114O26
- HSN Code
- 34024200
- Synonyms
- Tween 20, Polysorbate 20
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Nonionic surfactant, emulsifier, and stabilizer in biochemical applications.
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None identified.
3.1 — Substance
- Name
- Polysorbate 20
- CAS No.
- 9005-64-5
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Combustible material; may produce toxic fumes when burned.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate protective equipment. Clean up spills immediately to prevent slipping hazards.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal conditions of use.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Amber to pale yellow
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- ~100 °C
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.105 g/mL
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not specified
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions of use and storage.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; LD50 (oral, rat) > 5000 mg/kg.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Tween 20 Extra Pure is extensively used in the preparation of ELISA wash buffers, where it serves to reduce non-specific binding and improve assay sensitivity. Additionally, it is commonly employed in Western blotting as a membrane-blocking agent, helping to prevent non-specific interactions during protein detection. Beyond these applications, Tween 20 is also utilized in the formulation of pharmaceuticals, cosmetics, and food products, where it acts as an emulsifier and stabilizer. Its versatility makes it a valuable component in various industries, including biotechnology, pharmaceuticals, and food science.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01551
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 1227.72 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Golden yellow /amber color viscous liquid | Golden yellow color viscous liquid |
| Hydroxyl number | 97 - 113 | 99 |
| Saponification value | 40 - 51 | 41.2 |
| d20°/4°C | 1.105 - 1.107g | 1.106g |
The above product complies with EP grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Extra Pure | ≥ 99% | Not applicable | ≤ 1% | ≤ 0.5% | None detected | Soluble in water |
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