Polysorbate 20
- CAS Number
- 9005-64-5
- HSN Code
- 34024200
- Molecular Formula
- C58H114O26
- Catalogue Number
- AL1551A
- Purity
- ≥ 99%
- Molecular Weight
- 1227.72 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
TWEEN® 20 is a nonionic surfactant and emulsifier widely used in molecular biology applications. This specific grade is purified for use in sensitive assays such as ELISA and Western blotting, where high purity is essential to avoid interference with results. Its unique structure allows it to effectively reduce surface tension, making it an ideal choice for applications requiring solubilization of proteins and other biomolecules. TWEEN® 20 is known for its ability to stabilize emulsions and enhance the solubility of hydrophobic compounds, making it a versatile reagent in various laboratory settings.
Also known as TWEEN 20, Polysorbate 20.
- Catalogue No.
- AL1551A
- CAS Number
- 9005-64-5
- Molecular Formula
- C58H114O26
- Molecular Weight
- 1227.72 g/mol
- HSN Code
- 34024200
- Grade
- For Molecular Biology
- Product Form
- Substance
TWEEN® 20 appears as an amber to pale yellow viscous liquid. It has a density of 1.105 g/mL, indicating a relatively high viscosity compared to water. The product is soluble in water, forming clear solutions, and has a molecular weight of 1227.72 g/mol. The viscosity of TWEEN® 20 is significant, which contributes to its effectiveness as a surfactant in various applications. It is important to handle this product with care due to its viscous nature, which can affect dispensing and mixing processes.
- Appearance
- Amber to pale yellow viscous liquid
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- Polysorbate 20
- Catalogue No.
- AL1551A
- Product form
- Substance
- CAS Number
- 9005-64-5
- EC Number
- None available
- Grade
- For Molecular Biology
- EC Index Number
- None available
- Chemical Formula
- C58H114O26
- HSN Code
- 34024200
- Synonyms
- TWEEN 20, Polysorbate 20
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Nonionic surfactant and emulsifier in biological applications
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None known
3.1 — Substance
- Name
- Polysorbate 20
- CAS No.
- 9005-64-5
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Combustible material; may produce toxic fumes in a fire.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate protective equipment. Clean up spills immediately to prevent slipping hazards.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal conditions of use.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Amber to pale yellow
- Odour
- Mild
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- > 100 °C
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.105 g/mL
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions of use and storage.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; no significant adverse effects expected with proper handling.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not expected to be a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
TWEEN® 20 is primarily used in molecular biology as a nonionic detergent in ELISA wash buffers and Western blot blocking/washing buffer preparations. Its ability to solubilize proteins and reduce non-specific binding makes it invaluable in immunoassays. Additionally, it is utilized in cell culture applications to enhance the growth of cells by providing a stable environment. Beyond molecular biology, TWEEN® 20 is also employed in the pharmaceutical industry for drug formulation and in cosmetics as an emulsifier, showcasing its versatility across various fields.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1551A
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 1227.72 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay Purity | ≥ 99% | 99.5% |
| Moisture Content | ≤ 1% | 0.5% |
| Residue on Ignition | ≤ 0.5% | 0.3% |
| Related Substances | None detected | None detected |
| Solubility | Soluble in water | Clear solution |
The above product is expected to comply with For Molecular Biology grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| For Molecular Biology | ≥ 99% | Not applicable | ≤ 1% | ≤ 0.5% | None detected | Soluble in water |
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“From ordering to shipping, every course of action was smooth. Timely delivery.”
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