Ponceau S sodium salt
- CAS Number
- 6226-79-5
- HSN Code
- 32049000
- Molecular Formula
- C22H12N4Na4O13S4
- Catalogue Number
- AL01218
- Purity
- Min. 80%
- Molecular Weight
- 760.56 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Ponceau S sodium salt (certified, for molecular biology) is a red azo dye widely used as a reversible protein-detection stain in Western blotting. This compound is classified under the color index as C.I. No. 27195 and is known for its high dye content, with a minimum of 80%. It is particularly valued in molecular biology for its ability to provide a clear visual indication of protein transfer efficiency on membranes. The dye's molecular structure includes multiple sulfonate groups, which enhance its solubility in aqueous solutions, making it suitable for various laboratory applications.
Also known as C.I. 27195, Acid Red 113.
- Catalogue No.
- AL01218
- CAS Number
- 6226-79-5
- Molecular Formula
- C22H12N4Na4O13S4
- Molecular Weight
- 760.56 g/mol
- HSN Code
- 32049000
- Grade
- Certified
- Product Form
- Substance
Ponceau S sodium salt appears as a dark red powder. It is highly soluble in water, allowing for easy preparation of staining solutions. The dye exhibits a melting point of approximately 300 °C, and its solubility in water is around 10 g/L at room temperature. The compound has a density of about 1.5 g/cm³. Its strong color and solubility make it an effective choice for protein visualization in laboratory settings.
- Appearance
- Dark red powder
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place away from light.
1.1 — Product identifier
- Chemical Name
- Ponceau S sodium salt
- Catalogue No.
- AL01218
- Product form
- Substance
- CAS Number
- 6226-79-5
- EC Number
- None available
- Grade
- Certified
- EC Index Number
- None available
- Chemical Formula
- C22H12N4Na4O13S4
- HSN Code
- 32049000
- Synonyms
- C.I. 27195, Acid Red 113
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Reversible protein-detection stain
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None identified
3.1 — Substance
- Name
- Ponceau S sodium salt
- CAS No.
- 6226-79-5
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin, eyes, and respiratory tract.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- No specific hazards identified; use standard fire-fighting procedures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate methods. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Clean up spills immediately to prevent slipping hazards.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal conditions of use.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Approx. 300 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- Approx. 1.5 g/cm³
- Solubility
- Soluble in water (10 g/L at room temperature)
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions of use and storage.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; no specific data available.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not expected to be a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Ponceau S sodium salt is primarily used as a reversible total-protein stain for visualizing transfer efficiency on Western blot membranes. It is widely utilized in molecular biology laboratories for protein analysis, allowing researchers to confirm successful protein transfer from gels to membranes. Additionally, it can be used in various staining protocols for proteins in electrophoresis and is applicable in quality control processes in biopharmaceutical production. The dye's ability to bind to proteins makes it a valuable tool in proteomics and related fields.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01218
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 760.56 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay | Min. 80% | 82% |
| Melting Point | Approx. 300 °C | 302 °C |
| Solubility | Soluble in water | Soluble at 10 g/L |
The above product is expected to comply with Certified grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Certified | Min. 80% | Approx. 300 °C | Not specified | Not specified | Not specified | Soluble in water (10 g/L at room temperature) |
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