Pyronin B
- CAS Number
- 2150-48-3
- HSN Code
- 32049000
- Molecular Formula
- C42H54Cl8Fe2N4O2
- Catalogue Number
- AL1282A
- Purity
- Min. 30% dye content
- Molecular Weight
- 1042.22 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Pyronin B (C.I. No. 45010) is a synthetic xanthene dye primarily utilized in histological applications, particularly for RNA staining. This compound is notable for its ability to selectively bind to RNA, making it an essential tool in cytology and histology for visualizing nucleic acids. With a minimum dye content of 30%, Pyronin B is characterized by its dark red-brown powder form, which is indicative of its concentrated dye properties. Its molecular structure includes multiple chlorine and iron atoms, contributing to its unique staining capabilities and chemical behavior in biological samples.
Also known as Pyronin B, C.I. 45010.
- Catalogue No.
- AL1282A
- CAS Number
- 2150-48-3
- Molecular Formula
- C42H54Cl8Fe2N4O2
- Molecular Weight
- 1042.22 g/mol
- HSN Code
- 32049000
- Grade
- C.I. No. 45010
- Product Form
- Substance
Pyronin B appears as a dark red-brown powder, which is typical for many xanthene dyes. It has a molecular weight of 1042.22 g/mol and a molecular formula of C42H54Cl8Fe2N4O2. The dye is soluble in water and organic solvents, which facilitates its use in various staining protocols. The melting point of Pyronin B is not explicitly stated, but similar xanthene dyes typically exhibit melting points in the range of 200-300 °C. The density of the powder is approximately 1.5 g/cm³, which is common for such organic compounds. The dye is stable under normal conditions but should be stored in a cool, dry place to maintain its integrity.
- Appearance
- Dark red-brown powder
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Pyronin B
- Catalogue No.
- AL1282A
- Product form
- Substance
- CAS Number
- 2150-48-3
- EC Number
- Not specified
- Grade
- C.I. No. 45010
- EC Index Number
- Not specified
- Chemical Formula
- C42H54Cl8Fe2N4O2
- HSN Code
- 32049000
- Synonyms
- Pyronin B, C.I. 45010
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Dye for histological staining, specifically for RNA
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause respiratory irritation; avoid inhalation.
3.1 — Substance
- Name
- Pyronin B
- CAS No.
- 2150-48-3
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Seek medical attention if irritation persists.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of respiratory tract, skin, and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes under fire conditions; use appropriate fire-fighting measures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment. Avoid dust generation.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Sweep up and place in a suitable container for disposal. Avoid release into the environment.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Use gloves and a dust mask when handling to avoid inhalation and skin contact.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a dust mask or respirator if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red-brown
- Odour
- Odourless
- Odour threshold
- Not specified
- pH value
- Not specified
- Relative evaporation rate (butylacetate=1)
- Not specified
- Melting point
- Not specified
- Freezing point
- Not specified
- Boiling point
- Not specified
- Flash point
- Not specified
- Auto-ignition temperature
- Not specified
- Decomposition temperature
- Not specified
- Flammability (solid, gas)
- Not flammable under normal conditions
- Vapour pressure
- Not specified
- Relative vapour density at 20°C
- Not specified
- Density
- Approx. 1.5 g/cm³
- Solubility
- Soluble in water and organic solvents
- Log Pow
- Not specified
- Viscosity, kinematic
- Not specified
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not specified
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Not classified as acutely toxic; however, may cause irritation.
- Skin corrosion/irritation
- May cause skin irritation upon contact.
- Serious eye damage/irritation
- May cause eye irritation.
- Respiratory or skin sensitisation
- Not classified as a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Pyronin B is widely used in histology and cytology, particularly in the methyl green-pyronin staining technique, which differentiates between DNA and RNA in cellular samples. This application is crucial in research and clinical laboratories for the study of cell morphology and the assessment of RNA content in various tissues. Additionally, Pyronin B is employed in the field of molecular biology for visualizing RNA in gel electrophoresis and other analytical techniques. Its ability to selectively stain RNA makes it valuable in educational settings for teaching histological techniques and in research for studying gene expression and cellular activity.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1282A
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 1042.22 g/mol
| Test | Standard | Observed |
|---|---|---|
| Melting point | 174-176 0c | 175 0C |
| Absorption maxima | about 553 nm | 553.8 nm |
| Specific Absorptivity A 1% 1cm (? max; 0.0003%:ethanol 50%) | 1800 min | 1805.54 |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| C.I. No. 45010 | Min. 30% dye content | Not specified | Not specified | Not specified | Not specified | Soluble in water and organic solvents |
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