Safranine alcoholic staining solution
- CAS Number
- -
- HSN Code
- 38229090
- Molecular Formula
- C20H19ClN4
- Catalogue Number
- AL1298A
- Purity
- N/A
- Molecular Weight
- 368.84 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Safranine alcoholic staining solution is a ready-made alcohol-based preparation of the classic stain, safranine. This solution is primarily used in histology and plant tissue staining, providing a vibrant red color that enhances contrast in microscopic samples. The solution is formulated for immediate use, eliminating the need for dilution or preparation, which saves time in laboratory settings. Its alcohol base allows for rapid penetration and fixation of tissues, making it an essential tool in biological and medical research.
Also known as Safranine, Safranin O.
- Catalogue No.
- AL1298A
- CAS Number
- -
- Molecular Formula
- C20H19ClN4
- Molecular Weight
- 368.84 g/mol
- HSN Code
- 38229090
- Grade
- Ready-to-use alcoholic staining solution
- Product Form
- Mixture
The solution appears as a dark red liquid, indicative of the safranine dye dissolved in alcohol. It has a characteristic odor associated with alcohols. The density of the solution is approximately 0.8 g/cm³, and it has a boiling point around 78-82 °C due to the alcohol content. The solution is soluble in water and alcohol, making it versatile for various staining applications. The flash point is approximately 12 °C, highlighting its flammable nature.
- Appearance
- Dark red liquid
- Shelf Life
- 12 months
- Packaging
- Available in 100 mL and 500 mL bottles
- Storage
- Store in a cool, dry place away from heat and ignition sources.
1.1 — Product identifier
- Chemical Name
- Safranine alcoholic staining solution
- Catalogue No.
- AL1298A
- Product form
- Mixture
- CAS Number
- Not applicable
- EC Number
- Not applicable
- Grade
- Ready-to-use alcoholic staining solution
- EC Index Number
- Not applicable
- Chemical Formula
- C20H19ClN4
- HSN Code
- 38229090
- Synonyms
- Safranine, Safranin O
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Staining agent in histology and plant tissue analysis
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Flammable liquid and vapor; may cause eye irritation.
3.1 — Substance
- Name
- Safranine alcoholic staining solution
- CAS No.
- Not applicable
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Do not induce vomiting. Rinse mouth and seek medical attention.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; headache; dizziness.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Flammable; keep away from heat, sparks, open flames, and hot surfaces.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Evacuate area and eliminate all ignition sources. Ventilate area.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Use in a well-ventilated area. Avoid contact with skin and eyes. Wear appropriate personal protective equipment.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from heat and ignition sources.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if ventilation is inadequate.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Dark red
- Odour
- Alcoholic
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- 78-82 °C
- Flash point
- 12 °C
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Flammable liquid
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 0.8 g/cm³
- Solubility
- Soluble in water and alcohol
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not applicable
- Oxidising properties
- Not applicable
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions. Avoid heat, sparks, and open flames.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids, and bases.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- May be harmful if swallowed or inhaled; causes skin and eye irritation.
- Skin corrosion/irritation
- Causes mild skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- Not classified as a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- UN 1170
- UN-No. (IMDG)
- UN 1170
- UN-No. (IATA)
- UN 1170
- UN-No. (ADN)
- UN 1170
- UN-No. (RID)
- UN 1170
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Ethyl alcohol solution
- Proper Shipping Name (IMDG)
- Ethyl alcohol solution
- Proper Shipping Name (IATA)
- Ethyl alcohol solution
- Proper Shipping Name (ADN)
- Ethyl alcohol solution
- Proper Shipping Name (RID)
- Ethyl alcohol solution
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- 3
14.4 — Packing group
- Packing group (ADR)
- II
- Packing group (IMDG)
- II
- Packing group (IATA)
- II
- Packing group (ADN)
- II
- Packing group (RID)
- II
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Safranine alcoholic staining solution is widely used in histological and botanical studies as a counterstain. It is particularly effective in staining plant tissues, allowing for the visualization of cellular structures under a microscope. In histology, it is often used in conjunction with other stains to enhance the contrast of specific cellular components, such as nuclei and cytoplasm. Additionally, it finds applications in educational settings for teaching microscopy techniques and in research laboratories for various biological studies.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL1298A
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 368.84 g/mol
| Test | Standard | Observed |
|---|---|---|
| Appearance | Dark red liquid | Dark red liquid |
| Boiling Point | 78-82 °C | 80 °C |
| Density | 0.8 g/cm³ | 0.79 g/cm³ |
| Flash Point | 12 °C | 11 °C |
The above product is expected to comply with Ready-to-use alcoholic staining solution grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Ready-to-use alcoholic staining solution | N/A | 78-82 °C | N/A | N/A | N/A | Soluble in water and alcohol |
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