Safranine (M.S.)
- CAS Number
- 477-73-6
- HSN Code
- 32041310
- Molecular Formula
- C20H19ClN4
- Catalogue Number
- AL01298
- Purity
- Min. 80%
- Molecular Weight
- 350.84 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Safranine (M.S., C.I. No.50240) is a classic azine dye known for its vibrant dark red color and is widely utilized in histology and microbiology. This compound is particularly notable for its role as a counterstain in Gram staining, a critical technique for differentiating bacterial species. With a minimum dye content of 80%, it is a reliable choice for various staining applications. Its molecular structure, characterized by the presence of chlorine and nitrogen atoms, contributes to its unique staining properties and solubility in water, making it an essential tool in laboratory settings.
Also known as Safranin O, C.I. 50240.
- Catalogue No.
- AL01298
- CAS Number
- 477-73-6
- Molecular Formula
- C20H19ClN4
- Molecular Weight
- 350.84 g/mol
- HSN Code
- 32041310
- Grade
- M.S., C.I. No.50240
- Product Form
- Substance
Safranine appears as a dark red crystalline powder. It has a melting point of approximately 200 °C and is soluble in water, allowing for easy preparation of staining solutions. The density of safranine is around 1.2 g/cm³, and it has a relatively low vapor pressure, indicating stability under normal laboratory conditions. The compound has a characteristic odor, although it is not overpowering. Its solubility in water is a key factor that facilitates its use in various biological staining protocols.
- Appearance
- Dark red crystalline powder
- Shelf Life
- 2 years
- Packaging
- Available in 100 g and 500 g containers
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Safranine (M.S.)
- Catalogue No.
- AL01298
- Product form
- Substance
- CAS Number
- 477-73-6
- EC Number
- 207-202-4
- Grade
- M.S., C.I. No.50240
- EC Index Number
- Not applicable
- Chemical Formula
- C20H19ClN4
- HSN Code
- 32041310
- Synonyms
- Safranin O, C.I. 50240
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Dye for histological and microbiological applications
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause respiratory irritation; avoid inhalation.
3.1 — Substance
- Name
- Safranine (M.S.)
- CAS No.
- 477-73-6
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of the respiratory tract, skin, and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes in a fire; use appropriate fire-fighting measures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment and dispose of waste according to local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid inhalation and contact with skin; use gloves and a dust mask when handling.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a dust mask or respirator if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark red
- Odour
- Slightly aromatic
- Odour threshold
- Not established
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 200 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not established
- Flammability (solid, gas)
- Not flammable under normal conditions
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.2 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not established
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids, and bases.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; LD50 (oral, rat) > 2000 mg/kg.
- Skin corrosion/irritation
- May cause mild skin irritation.
- Serious eye damage/irritation
- May cause mild eye irritation.
- Respiratory or skin sensitisation
- Not a skin sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- No specific target organ toxicity.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Safranine is primarily used as a counterstain in Gram staining, which is essential for identifying and classifying bacteria based on their cell wall composition. In addition to its microbiological applications, it serves as a general histological stain for cellulose and lignified plant tissues, aiding in the visualization of plant structures under a microscope. Furthermore, safranine is utilized in various biological and chemical research applications, including studies of cell morphology and tissue structure. Its vibrant color also makes it useful in educational settings for teaching purposes in biology and histology.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01298
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 350.84 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark Greenish colored powder | Dark Greenish colored powder |
| Absorption Maxima in (50% ethanol) | 530 - 534 nm | 532 nm |
| Specific Absorptivity (A 1%/1cm at ? max) | 1260 - 1570 | 1510 |
| Loss on drying at 110°C | <15.0% | Passes test |
| Suitability for microscopy | Passes test |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| M.S. | Min. 80% | 200 °C | Max. 5% | Max. 1% | None detected | Soluble in water |
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