Thionin acetate salt
- CAS Number
- 78338-22-4
- HSN Code
- 32049000
- Molecular Formula
- C14H13N3O2S
- Catalogue Number
- AL01503
- Purity
- Min. 85% dye content
- Molecular Weight
- 287.34 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Thionin acetate salt (M.S., C.I. No.52000) is a classic thiazine dye known for its application in histology, particularly in the Nissl staining technique used to visualize neuronal cell bodies. This compound is characterized by its dark purple-brown crystalline powder form, which is indicative of its thiazine structure. With a minimum dye content of 85%, it is a reliable choice for researchers in neuroscience and histopathology. The molecular weight of 287.34 g/mol and the specific CAS number 78338-22-4 further identify this compound as a significant tool in biological staining applications.
Also known as Thionin, Thionin acetate, C.I. 52000.
- Catalogue No.
- AL01503
- CAS Number
- 78338-22-4
- Molecular Formula
- C14H13N3O2S
- Molecular Weight
- 287.34 g/mol
- HSN Code
- 32049000
- Grade
- M.S., C.I. No.52000
- Product Form
- Substance
Thionin acetate salt appears as a dark purple-brown crystalline powder. It has a melting point of approximately 200 °C, which is typical for many thiazine dyes. The compound is soluble in water, allowing for easy preparation of staining solutions. Its density is around 1.3 g/cm³, which is consistent with similar organic compounds. The dye exhibits a characteristic odor, although it is not overpowering, and it is stable under normal laboratory conditions.
- Appearance
- Dark purple-brown crystalline powder
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place away from light.
1.1 — Product identifier
- Chemical Name
- Thionin acetate salt
- Catalogue No.
- AL01503
- Product form
- Substance
- CAS Number
- 78338-22-4
- EC Number
- Not specified
- Grade
- M.S., C.I. No.52000
- EC Index Number
- Not specified
- Chemical Formula
- C14H13N3O2S
- HSN Code
- 32049000
- Synonyms
- Thionin, Thionin acetate, C.I. 52000
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Dye for histological staining
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause respiratory irritation; avoid inhalation.
3.1 — Substance
- Name
- Thionin acetate salt
- CAS No.
- 78338-22-4
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of the respiratory tract, skin, and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes when burned.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment and dispose of waste according to local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid inhalation and contact with skin. Use gloves and a dust mask when handling.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from light and incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use a dust mask or respirator if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark purple-brown
- Odour
- Slight
- Odour threshold
- Not specified
- pH value
- Not specified
- Relative evaporation rate (butylacetate=1)
- Not specified
- Melting point
- 200 °C
- Freezing point
- Not specified
- Boiling point
- Not specified
- Flash point
- Not specified
- Auto-ignition temperature
- Not specified
- Decomposition temperature
- Not specified
- Flammability (solid, gas)
- Not flammable under normal conditions
- Vapour pressure
- Not specified
- Relative vapour density at 20°C
- Not specified
- Density
- 1.3 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not specified
- Viscosity, kinematic
- Not specified
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not specified
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Not classified as acutely toxic.
- Skin corrosion/irritation
- May cause skin irritation.
- Serious eye damage/irritation
- May cause eye irritation.
- Respiratory or skin sensitisation
- Not classified as a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Thionin acetate salt is primarily used in histology for Nissl staining, which is essential for visualizing neuronal cell bodies and RNA in brain tissue sections. This dye is widely utilized in neuroscience research to study the morphology of neurons and their distribution in various brain regions. Additionally, it finds applications in the staining of other biological tissues, aiding in the identification of cellular structures in histopathological examinations. Its use extends to educational settings for teaching histological techniques and in laboratories focused on neurobiology and cellular biology.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01503
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 287.34 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Black or blackish green color powder | Blackish green color powder. |
| Dye Content | 85% min | 92.5 % |
| Absorption maxima in water | 598 - 605nm | 600.10 nm |
| Absorptivity A1% / 1 cm in water | 1740 - 2040 | 1752.08 |
| Loss on drying(at 110 0C) | 7.0% max | 4.08% |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| M.S., C.I. No.52000 | Min. 85% dye content | 200 °C | Not specified | Not specified | Not specified | Soluble in water |
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