Toluidine Blue Solution
- CAS Number
- -
- HSN Code
- 38229090
- Molecular Formula
- C15H16N3S
- Catalogue Number
- AL01532
- Purity
- N/A
- Molecular Weight
- 273.37 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Toluidine blue solution is a ready-made preparation of the dye toluidine blue, which is a thiazine dye known for its metachromatic properties. This solution is primarily used in histological staining and as a vital dye in various biological applications. It is notable for its ability to stain nucleic acids and other cellular components, making it a valuable tool in microscopy and histopathology. The solution is prepared for immediate use, eliminating the need for further dilution or preparation, which enhances its convenience in laboratory settings.
Also known as Toluidine blue O, TBO.
- Catalogue No.
- AL01532
- CAS Number
- -
- Molecular Formula
- C15H16N3S
- Molecular Weight
- 273.37 g/mol
- HSN Code
- 38229090
- Grade
- Ready-to-use indicator solution
- Product Form
- Mixture
Toluidine blue solution appears as a dark blue liquid. It has a density of approximately 1.0 g/cm³ and is soluble in water, which allows for easy application in staining procedures. The solution has a pH range of about 4.5 to 6.0, making it suitable for various biological samples. The boiling point of toluidine blue is around 300 °C, and it does not have a defined melting point as it is a solution. The solution is stable under normal laboratory conditions and should be stored in a cool, dark place to maintain its efficacy.
- Appearance
- Dark blue liquid
- Shelf Life
- 12 months
- Packaging
- Available in 100 mL and 500 mL bottles
- Storage
- Store in a cool, dark place, away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- Toluidine Blue Solution
- Catalogue No.
- AL01532
- Product form
- Mixture
- CAS Number
- Not applicable
- EC Number
- N/A
- Grade
- Ready-to-use indicator solution
- EC Index Number
- N/A
- Chemical Formula
- C15H16N3S
- HSN Code
- 38229090
- Synonyms
- Toluidine blue O, TBO
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Staining and indicator in histology and cytology
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- May cause skin and eye irritation
3.1 — Substance
- Name
- Toluidine Blue Solution
- CAS No.
- Not applicable
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing. Seek medical attention if irritation persists.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of skin and eyes, respiratory discomfort if inhaled in large quantities.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Non-flammable; however, may emit toxic fumes when heated.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and absorb with inert material. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Clean with water and absorbent material. Dispose of according to local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dark place, tightly closed.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal use; use in well-ventilated areas.
9.1 — Information on basic physical and chemical properties
- Physical state
- Liquid
- Colour
- Dark blue
- Odour
- Slightly aromatic
- Odour threshold
- Not determined
- pH value
- 4.5 - 6.0
- Relative evaporation rate (butylacetate=1)
- Not determined
- Melting point
- Not applicable
- Freezing point
- Not applicable
- Boiling point
- Approx. 300 °C
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not determined
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not determined
- Relative vapour density at 20°C
- Not determined
- Density
- Approx. 1.0 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not determined
- Viscosity, kinematic
- Not determined
- Viscosity, dynamic
- Not determined
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; avoid strong oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents, strong acids.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; may cause irritation.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause serious eye irritation.
- Respiratory or skin sensitisation
- Not expected to be a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Toluidine blue solution is widely used in histology as a metachromatic stain, allowing for the differentiation of various tissue components based on their chemical properties. It is particularly useful in identifying nucleic acids, making it a critical tool in cancer research and diagnostics, especially in oral cancer screening. Additionally, it is employed in microbiology for staining bacteria and fungi, enhancing visibility under a microscope. The solution is also utilized in educational settings for teaching purposes, demonstrating staining techniques and cellular structures.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01532
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 273.37 g/mol
| Test | Standard | Observed |
|---|---|---|
| Appearance | Dark blue liquid | Dark blue liquid |
| pH | 4.5 - 6.0 | 5.0 |
| Density | Approx. 1.0 g/cm³ | 1.02 g/cm³ |
| Boiling Point | Approx. 300 °C | 295 °C |
The above product is expected to comply with Ready-to-use indicator solution grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Ready-to-use indicator solution | N/A | N/A | N/A | N/A | N/A | Soluble in water |
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