Tris(hydroxymethyl)aminomethane
- CAS Number
- 77-86-1
- HSN Code
- 29225090
- Molecular Formula
- C4H11NO3
- Catalogue Number
- AL01543
- Purity
- 99%
- Molecular Weight
- 121.14 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Tris buffer extra pure (tris base, tris(hydroxymethyl)aminomethane) is one of the most widely used biological buffers in molecular biology and biochemistry. It is particularly valued for its ability to maintain a stable pH in biological systems, making it essential for various laboratory applications. Tris buffer is often utilized in the preparation of electrophoresis buffers and protein solutions, where precise pH control is critical. Its high purity level of 99% ensures minimal interference in sensitive biochemical assays, making it a preferred choice for researchers and laboratories.
Also known as Tris base, tris(hydroxymethyl)aminomethane.
- Catalogue No.
- AL01543
- CAS Number
- 77-86-1
- Molecular Formula
- C4H11NO3
- Molecular Weight
- 121.14 g/mol
- HSN Code
- 29225090
- Grade
- Extra Pure
- Product Form
- Substance
Tris buffer appears as a white crystalline powder that is hygroscopic in nature. It is highly soluble in water, with a solubility of approximately 50 g/L at 25 °C. The melting point of Tris base is around 171 °C, and it has a pH range of 7.0 to 9.0 in solution, depending on concentration. The compound has a density of approximately 1.1 g/cm³. Tris buffer is stable under normal conditions but should be stored in a cool, dry place to prevent moisture absorption.
- Appearance
- White crystalline powder
- Shelf Life
- -
- Packaging
- -
- Storage
- Store in a cool, dry place, away from moisture.
1.1 — Product identifier
- Chemical Name
- Tris(hydroxymethyl)aminomethane
- Catalogue No.
- AL01543
- Product form
- Substance
- CAS Number
- 77-86-1
- EC Number
- 201-064-4
- Grade
- Extra Pure
- EC Index Number
- Not specified
- Chemical Formula
- C4H11NO3
- HSN Code
- 29225090
- Synonyms
- Tris base, tris(hydroxymethyl)aminomethane
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Biological buffer in molecular biology and biochemistry
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None identified
3.1 — Substance
- Name
- Tris(hydroxymethyl)aminomethane
- CAS No.
- 77-86-1
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin, eyes, and respiratory tract.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- Non-flammable; however, may produce toxic fumes in a fire.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect for disposal. Avoid release into the environment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate protective equipment. Sweep up and place in a suitable container for disposal.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from moisture and incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal conditions; use in a well-ventilated area.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- White
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- 7.0 - 9.0 (in solution)
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 171 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not specified
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.1 g/cm³
- Solubility
- 50 g/L in water at 25 °C
- Log Pow
- Not specified
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not specified
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions; incompatible with strong acids and oxidizing agents.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong acids, strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; LD50 (oral, rat) > 5000 mg/kg.
- Skin corrosion/irritation
- Mild irritant.
- Serious eye damage/irritation
- Mild irritant.
- Respiratory or skin sensitisation
- Not a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Tris buffer is extensively used in molecular biology and biochemistry, serving as a fundamental buffer in TAE (Tris-Acetate-EDTA) and TBE (Tris-Borate-EDTA) electrophoresis buffers. It is also employed in Tris-HCl protein buffers, which are crucial for maintaining protein stability during purification and analysis. Additionally, Tris buffer is utilized in various applications such as DNA and RNA extraction, enzyme assays, and cell culture media preparation. Its versatility and effectiveness in maintaining pH make it a staple in laboratories across the life sciences and biotechnology sectors.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01543
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 121.14 g/mol
| Test | Standard | Observed |
|---|---|---|
| Assay Purity | 99% min | 99.5% |
| Moisture Content | <0.5% | 0.3% |
| Residue on Ignition | <0.1% | 0.05% |
| Related Substances | None detected | None detected |
| Solubility | 50 g/L in water at 25 °C | 52 g/L in water at 25 °C |
The above product is expected to comply with Extra Pure grade specification.
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Extra Pure | 99% | 171 °C | <0.5% | <0.1% | None detected | 50 g/L in water at 25 °C |
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