Trypan Blue
- CAS Number
- 72-57-1
- HSN Code
- 32049000
- Molecular Formula
- C34H24N6O14S4Na4
- Catalogue Number
- AL01546
- Purity
- Min. 70%
- Molecular Weight
- 960.80 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Trypan blue (M.S., C.I. No.23850) is a vital dye extensively utilized in biological and medical research for its ability to differentiate between live and dead cells. This compound is particularly significant in cell viability assays, where it selectively stains non-viable cells, allowing researchers to assess cell health and proliferation. With a minimum dye content of 70%, it is a reliable choice for laboratories requiring precise and accurate cell counting. Its molecular structure, comprising multiple functional groups, contributes to its solubility and staining properties, making it a staple in cell biology.
Also known as C.I. 23850, Trypan Blue Stain.
- Catalogue No.
- AL01546
- CAS Number
- 72-57-1
- Molecular Formula
- C34H24N6O14S4Na4
- Molecular Weight
- 960.80 g/mol
- HSN Code
- 32049000
- Grade
- M.S., C.I. No.23850
- Product Form
- Substance
Trypan blue appears as a dark blue-black powder and is highly soluble in water, forming a blue solution. The melting point of trypan blue is approximately 200 °C, and it has a boiling point of around 100 °C when dissolved in water. The density of trypan blue is approximately 1.5 g/cm³. It has a pH value in solution of around 7, making it neutral and compatible with biological systems. The compound has a relative vapor density of 1.0, indicating it is not volatile under standard conditions.
- Appearance
- Dark blue-black powder
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g containers
- Storage
- Store in a cool, dry place, away from light.
1.1 — Product identifier
- Chemical Name
- Trypan Blue
- Catalogue No.
- AL01546
- Product form
- Substance
- CAS Number
- 72-57-1
- EC Number
- 200-586-2
- Grade
- M.S., C.I. No.23850
- EC Index Number
- Not applicable
- Chemical Formula
- C34H24N6O14S4Na4
- HSN Code
- 32049000
- Synonyms
- C.I. 23850, Trypan Blue Stain
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Cell viability staining in biological assays
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- Suspected carcinogen and teratogen; handle with care.
3.1 — Substance
- Name
- Trypan Blue
- CAS No.
- 72-57-1
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing and seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- Irritation of eyes, skin, and respiratory tract; potential long-term health effects.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- May emit toxic fumes under fire conditions.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate protective equipment.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use absorbent material to collect spill and dispose of according to local regulations.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use gloves and protective eyewear.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Use appropriate respiratory protection if dust is generated.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark blue-black
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- 7 (in solution)
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 200 °C
- Freezing point
- Not applicable
- Boiling point
- 100 °C (in solution)
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- 1.0
- Density
- 1.5 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Low toxicity; LD50 (oral, rat) > 2000 mg/kg.
- Skin corrosion/irritation
- May cause skin irritation.
- Serious eye damage/irritation
- Causes serious eye irritation.
- Respiratory or skin sensitisation
- Not a skin sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not classified as dangerous goods.
- UN-No. (IMDG)
- Not classified as dangerous goods.
- UN-No. (IATA)
- Not classified as dangerous goods.
- UN-No. (ADN)
- Not classified as dangerous goods.
- UN-No. (RID)
- Not classified as dangerous goods.
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not applicable
- Proper Shipping Name (IMDG)
- Not applicable
- Proper Shipping Name (IATA)
- Not applicable
- Proper Shipping Name (ADN)
- Not applicable
- Proper Shipping Name (RID)
- Not applicable
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Trypan blue is predominantly used in cell biology for assessing cell viability in various applications, including hemocytometer assays, where it is employed to count live and dead cells in culture. It is also utilized in histology for staining tissues and in microbiology for distinguishing viable microorganisms. Additionally, trypan blue is used in toxicology studies to evaluate the effects of various substances on cell health. Its role in research extends to drug development, where it helps in determining the cytotoxicity of new compounds. Furthermore, it is used in veterinary medicine for similar purposes in animal cell studies.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01546
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 960.80 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark blue crystalline powder | Dark blue crystalline powder |
| Dye content | Min. 70.0% | 71.2% |
| Absorption maxima (in water) | 590 - 600 nm | 600 nm |
| Spec. Absorptivity a1%/1cm max; 0.01g/l in water) | 650 - 820 | 759 |
| Loss on drying (110°C) | <10% | 9.1% |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| M.S. | Min. 70% | 200 °C | Max. 5% | Max. 1% | None detected | Soluble in water |
Related Products
View AllWhy buy from Alpha Chemika
What Our Scientific & Industrial Customers Say
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“One shop stop for all laboratory chemicals requirements.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“Exactly as advertised.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Alpha Chemika products are of excellent quality. Highly satisfied.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“From ordering to shipping, every course of action was smooth. Timely delivery.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“Completely satisfied with the products and services.”
“Speedy delivery, professional staff.”
“One shop stop for all laboratory chemicals requirements.”
FAQs
Common Questions About Laboratory Chemicals & Solutions
Find answers to common questions about laboratory chemicals, product selection, documentation, ordering, and technical support. Whether you are sourcing chemicals for research, testing, education, manufacturing, or industrial applications, these FAQs can help you make informed purchasing decisions.
Alpha Chemika offers a wide range of laboratory chemicals, reagents, solvents, and specialty products for research, testing, educational, pharmaceutical, and industrial applications.
Product selection depends on factors such as application, required purity, grade, specifications, and intended use. Our team can help you identify suitable products based on your technical requirements.
Yes. Relevant product information and technical documentation can be provided to help you evaluate products for quality, handling, compliance, and application requirements.
Yes. You can share your product list, required quantities, specifications, and delivery requirements with our team for a tailored quotation.
Yes. We support requirements from research laboratories, educational institutions, pharmaceutical companies, manufacturers, distributors, and other scientific and industrial organizations.
Connect with Us for Your Requirements
Have a specific product requirement or need help finding the right chemical for your application? Share your requirements with our team, and we will help you identify suitable products, specifications, quantities, and procurement options.
