Xylene Cyanol FF
- CAS Number
- 2650-17-1
- HSN Code
- 32041979
- Molecular Formula
- C25H27N2NaO6S2
- Catalogue Number
- AL01582
- Purity
- Min. 75%
- Molecular Weight
- 538.61 g/mol
Complies with : IP | BP | USP | EP | ACS
ISO 9001
ISO 14001
GMP
COA with every batch
Key Properties
Xylene cyanol FF extra pure (C.I. No.42135) is a synthetic dye that is primarily utilized as a tracking dye in gel electrophoresis, particularly in the analysis of nucleic acids. This compound is notable for its ability to provide a visual marker during the separation of DNA and RNA fragments, allowing researchers to monitor the progress of electrophoresis. With a minimum dye content of 75%, it is classified as extra pure, ensuring high performance in laboratory applications. The compound's molecular structure includes a sulfonate group, which enhances its solubility in aqueous solutions, making it ideal for use in various biological assays.
Also known as C.I. No.42135, Xylene Cyanol, C.I. 42135.
- Catalogue No.
- AL01582
- CAS Number
- 2650-17-1
- Molecular Formula
- C25H27N2NaO6S2
- Molecular Weight
- 538.61 g/mol
- HSN Code
- 32041979
- Grade
- Extra Pure
- Product Form
- Substance
Xylene cyanol FF appears as a dark blue-green powder. It is soluble in water, which is a critical property for its application in gel electrophoresis. The compound has a melting point of approximately 200 °C, and its solubility in water is significant, allowing for easy preparation of loading buffers. The density of xylene cyanol FF is approximately 1.2 g/cm³, and it has a relatively low vapor pressure, making it stable under normal laboratory conditions. The compound does not have a distinct odor, which is advantageous for laboratory use.
- Appearance
- Dark blue-green powder
- Shelf Life
- 2 years
- Packaging
- Available in 25 g and 100 g bottles
- Storage
- Store in a cool, dry place, away from direct sunlight.
1.1 — Product identifier
- Chemical Name
- Xylene Cyanol FF
- Catalogue No.
- AL01582
- Product form
- Substance
- CAS Number
- 2650-17-1
- EC Number
- 220-200-0
- Grade
- Extra Pure
- EC Index Number
- Not applicable
- Chemical Formula
- C25H27N2NaO6S2
- HSN Code
- 32041979
- Synonyms
- C.I. No.42135, Xylene Cyanol, C.I. 42135
1.2 — Relevant identified uses of the substance or mixture and uses advised against
- Use of the substance/mixture
- Tracking dye in gel electrophoresis
- Uses advised against
- Not specified
1.3 — Details of the supplier of the safety data sheet
- Company identification
- Address:- 102, B wing, Savgan Heights, RTO Lane,Andheri (W), Mumbai-400 053Maharashtra, India.
1.4 — Emergency Contact Details
- Phone / e-Mail
- Phone : +91 22 26317055 Mobile : +91 9820385757 e-mail : info@alphachemika.co.in
2.3 — Other hazards
- Other hazards
- None identified
3.1 — Substance
- Name
- Xylene Cyanol FF
- CAS No.
- 2650-17-1
4.1 — Description of first aid measures
- First-aid measures after inhalation
- Move to fresh air. If symptoms persist, seek medical attention.
- First-aid measures after skin contact
- Wash with soap and water. If irritation occurs, seek medical attention.
- First-aid measures after eye contact
- Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. If irritation persists, seek medical attention.
- First-aid measures after ingestion
- Rinse mouth. Do not induce vomiting. Seek medical attention if symptoms occur.
4.2 — Most important symptoms and effects, both acute and delayed
- Symptoms relating to use
- May cause mild irritation to skin and eyes.
4.3 — Indication of any immediate medical attention and special treatment needed
- General information
- Treat symptomatically. Show this safety data sheet to the treating physician.
5.1 — Extinguishing media
- Suitable extinguishing media
- Water spray, carbon dioxide, dry chemical powder, foam.
- Unsuitable extinguishing media
- Not specified
5.2 — Special hazards arising from the substance or mixture
- Special procedures
- No special hazards identified; use standard fire-fighting procedures.
5.3 — Advice for firefighters
- Protection during firefighting
- Do not enter fire area without proper protective equipment, including respiratory protection.
6.1.1 — For non-emergency personnel
- Emergency procedures
- Contain spill and collect using appropriate methods. Dispose of in accordance with local regulations.
6.3 — Methods and material for containment and cleaning up
- Methods for cleaning up
- Use appropriate personal protective equipment. Clean up spills immediately to prevent slipping hazards.
6.4 — Reference to other sections
- Reference
- Not specified
7.1 — Precautions for safe handling
- Precautions for safe handling
- Avoid contact with skin and eyes. Use in a well-ventilated area.
- Hygiene measures
- Wash hands and other exposed areas with mild soap and water before eating, drinking or smoking and when leaving work.
7.2 — Conditions for safe storage, including any incompatibilities
- Storage conditions
- Store in a cool, dry place, away from incompatible materials.
8.2 — Exposure controls
- Hand protection
- Protective gloves (e.g. nitrile rubber)
- Eye protection
- Chemical safety goggles or safety glasses
- Skin and body protection
- Wear suitable protective clothing
- Respiratory protection
- Not required under normal conditions of use; use in well-ventilated areas.
9.1 — Information on basic physical and chemical properties
- Physical state
- Solid
- Colour
- Dark blue-green
- Odour
- Odourless
- Odour threshold
- Not applicable
- pH value
- Not applicable
- Relative evaporation rate (butylacetate=1)
- Not applicable
- Melting point
- 200 °C
- Freezing point
- Not applicable
- Boiling point
- Not applicable
- Flash point
- Not applicable
- Auto-ignition temperature
- Not applicable
- Decomposition temperature
- Not applicable
- Flammability (solid, gas)
- Not flammable
- Vapour pressure
- Not applicable
- Relative vapour density at 20°C
- Not applicable
- Density
- 1.2 g/cm³
- Solubility
- Soluble in water
- Log Pow
- Not applicable
- Viscosity, kinematic
- Not applicable
- Viscosity, dynamic
- Not applicable
- Explosive properties
- Not explosive
- Oxidising properties
- Not oxidizing
- Explosive limits
- Not applicable
10.1 — Reactivity
- Reactivity
- Stable under normal conditions of use and storage.
10.2 — Chemical stability
- Chemical stability
- Stable under recommended storage conditions.
10.3 — Possibility of hazardous reactions
- Possibility of hazardous reactions
- No dangerous reactions known under normal conditions of use.
10.4 — Conditions to avoid
- Conditions to avoid
- Avoid contact with incompatible materials and excessive heat.
10.5 — Incompatible materials
- Incompatible materials
- Strong oxidizing agents.
10.6 — Hazardous decomposition products
- Hazardous decomposition products
- Not specified
11.1 — Information on toxicological effects
- Acute toxicity
- Not classified as acutely toxic.
- Skin corrosion/irritation
- May cause mild irritation.
- Serious eye damage/irritation
- May cause mild irritation.
- Respiratory or skin sensitisation
- Not classified as a sensitizer.
- Germ cell mutagenicity
- Not classified
- Carcinogenicity
- Not classified
- Specific target organ toxicity (single exposure)
- Not classified as a specific target organ toxicant.
- Specific target organ toxicity (repeated exposure)
- Not classified
12.1 — Toxicity
- Toxicity information
- Not specified
12.2 — Persistence - degradability
- Persistence - degradability
- Not specified
12.3 — Bioaccumulative potential
- Bioaccumulative potential
- Not specified
12.4 — Mobility in soil
- Mobility in soil
- Not specified
12.6 — Other adverse effects
- Environmental precautions
- Not specified
13.1 — Waste treatment methods
- Additional Info
- Dispose of contents/container in accordance with local, regional, national and international regulations.
14.1 — UN number
- UN-No. (ADR)
- Not assigned
- UN-No. (IMDG)
- Not assigned
- UN-No. (IATA)
- Not assigned
- UN-No. (ADN)
- Not assigned
- UN-No. (RID)
- Not assigned
14.2 — UN proper shipping name
- Proper Shipping Name (ADR)
- Not classified as dangerous goods
- Proper Shipping Name (IMDG)
- Not classified as dangerous goods
- Proper Shipping Name (IATA)
- Not classified as dangerous goods
- Proper Shipping Name (ADN)
- Not classified as dangerous goods
- Proper Shipping Name (RID)
- Not classified as dangerous goods
14.3 — Transport hazard class(es)
- Transport hazard class(es)
- Not applicable
14.4 — Packing group
- Packing group (ADR)
- Not applicable
- Packing group (IMDG)
- Not applicable
- Packing group (IATA)
- Not applicable
- Packing group (ADN)
- Not applicable
- Packing group (RID)
- Not applicable
14.5 — Environmental hazards
- Dangerous for the environment
- Not specified
- Marine pollutant
- Not specified
- Other information
- No supplementary information available
15.1 — Safety, health and environmental regulations/legislation specific for the substance or mixture
- 15.1.1 EU-Regulations
- Not specified
Xylene cyanol FF is extensively used in molecular biology laboratories as a tracking dye in agarose and polyacrylamide gel-loading buffers for DNA and RNA electrophoresis. Its slow migration rate allows for effective monitoring of nucleic acid separation during electrophoresis, making it a valuable tool in genetic research, forensic analysis, and quality control of nucleic acid preparations. Additionally, it is used in various staining protocols and can be employed in the visualization of proteins in gel electrophoresis. The dye is also utilized in educational settings for teaching electrophoresis techniques.
ISO 9001
ISO 14001
GMP
COA with every batch
- Batch Number
- AL-2026-AL01582
- Manufacturing Date
- September-2026
- Expiry Date
- September-2031
- Molecular Weight
- 538.61 g/mol
| Test | Standard | Observed |
|---|---|---|
| Description | Dark green crystalline powder with metallic luster | Dark green crystalline powder with metallic luster |
| Suitability as redox indicator | Passes test | Passes test |
| Absorption maxima | About 615 nm | 613 nm |
The above product complies with LR grade
Technical Data Sheet
| Grade | Assay/Purity | Melting/Boiling Point | Moisture/LOD | Residue on Ignition | Related Substances | Solubility |
|---|---|---|---|---|---|---|
| Extra Pure | Min. 75% | 200 °C | Max. 1% | Max. 0.5% | None detected | Soluble in water |
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